BDNF antibody

Catalog No. ABIN115985

Product Details anti-BDNF Antibody

Target: BDNF (Brain-Derived Neurotrophic Factor (BDNF))

Reactivity: Human, Rat, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This BDNF antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Frozen Sections) (IHC (fro)), Enzyme Immunoassay (EIA), Functional Studies (Func)

Specificity: The antibody recognises BDNF.

Cross-Reactivity (Details): Species reactivity (tested):Human, Mouse and Rat.

Purification: Affinity Chromatography employing immobilized Human BDNF matrix

Immunogen: Highly purified (>98%) E.coli derived recombinant Human BDNF (Cat.-No PA047)

Application Details

Application Notes: ELISA: Indirect To detect hBDNF by indirect ELISA (using 100 μL/well antibody solution) aconcentration of 0.5 - 2.0 μg/mL of this antibody is required. This antibody, in conjunctionwith compatible secondary reagents, allows the detection of at least 0.2 - 0.4 ng/well ofrecombinant hBDNF. Sandwich To detect hBDNF by sandwich ELISA (using 100 μL/well antibody solution) aconcentration of 0.5 - 2.0 μg/mL of this antibody is required. This antibody, in conjunctionwith a biotinylated anti-Human BDNF (PP1000B1 / PP1000B2) as a detection antibody,allows the detection of at least 0.2 - 0.4 ng/well of recombinant hBDNF. Western blot: To detect hBDNF by Western blot analysis this antibody can be used at a concentration of0.1 - 0.2 μg/mL. The detection limit for recombinant hBDNF is 1.5 - 3.0 ng/lane, under eitherreducing or non-reducing conditions.

Protocol: NEUROTROPHIN IMMUNOHISTOCHEMISTRYThe neurotrophins have proved difficult to localize which may be due to masking by, forexample, their association with the trk receptors or very low concentrations. Wheresuccess has been achieved, the conditions required vary greatly for different tissues. Aprotocol has been included for central nervous system tissue and, while it is similar tocommonly used methods, it is important to give strict attention to details such as thoroughwashing, fixative and detergent concentrations, concentration and quality of the primaryantibodies and length of incubations, etc. You may find it possible to use alternateprotocols, however we have experienced many failures using variations of the currentprotocol (and some failures when strict adherence to the procedure is maintained). Asuitable procedure to stain nerve terminals is still being developed. Neurotrophinreceptors have proved much easier to localize. It is recommended that you include a few sections of adult rat cerebellum, spinal cord orkidney in each experiment since these are tissues which are the easiest to stain. Protocol for Immunohistochemistry in the Central Nervous System(At all steps thorough washing is necessary to reduce background)Fixation: Animals are perfused with 1% sodium nitrite in phosphate buffered saline (PBS) (about 50ml) followed by 1 liter of Zamboni's fixative (4% formaldehyde, 15% picric acid in 0. 1Mphosphate buffer). Post fix for no longer than 2 hours. Tissue Preparation: Tissues are removed and washed briefly with PBS followed by cryoprotection in 30%sucrose in PBS overnight at 4°C. 30 µm cryostat sections are cut and washed with agitationin: PBS (1x15min)50% ethanol (3x15min)

Restrictions: For Research Use only

Handling

Reconstitution: Restore in sterile water to a concentration of 0.1-1.0 mg/mL.

Buffer: PBS, pH 7.2, without preservatives

Preservative: Without preservative

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Prior to reconstitution store at 2-8 °C. Following reconstitution store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

References for anti-BDNF antibody (ABIN115985)

Hartog, Dittrich, Pieneman, Jansen, Frankl-Vilches, Lessmann, Lilliehook, Goldman, Gahr: "Brain-derived neurotrophic factor signaling in the HVC is required for testosterone-induced song of female canaries." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 29, Issue 49, pp. 15511-9, (2009) (PubMed).

Target Details for BDNF

Target: BDNF (Brain-Derived Neurotrophic Factor (BDNF))

Alternative Name: BDNF (BDNF Products)

Synonyms: ANON2 antibody, BULN2 antibody, bdnf-A antibody, BDNF antibody, bdnf antibody, brain derived neurotrophic factor antibody, brain-derived neurotrophic factor antibody, brain-derived neurotrophic factor L homeolog antibody, brain-derived neurotrophic factor S homeolog antibody, BDNF antibody, Bdnf antibody, bdnf antibody, bdnf.L antibody, bdnf.S antibody

Background: Brain derived neurotrophic factor (BDNF) is a member of the neurotrophin family of growth factors that includes NGF, NT3, and NT4. All neurotrophins have six conserved cysteine residues and share a 55 % sequence identity at the amino acid level. BDNF is a potent neurotrophic factor that supports the growth and survivability of nerve and/or glial cells. BDNF has been shown to enhance the survival and differentiation of several classes of neurons in vitro, including neural crest and placode derived sensory neurons, dopaminergic neurons in the substantia nigra, basal forebrain cholinergic neurons, hippocampal neurons, and retinal ganglial cells. BDNF is expressed within peripheral ganglia and is not restricted to neuronal target fields, raising the possibility that BDNF has paracrine or even autocrine actions on neurons as well as non neuronal cells. Expression of BDNF is reduced in both Alzheimer's and Huntington disease patients. In addition, functional studies showed that age-associated changes in BDNF-mediated pathways can enhance inflammation and increase myocardial injury after myocardial infarction in the aging heart.Synonyms: Abrineurin, Brain-derived neurotrophic factor

Gene ID: 627

NCBI Accession: NP_001137277

UniProt: P23560

Pathways: RTK Signaling, Synaptic Membrane, Feeding Behaviour, Dicarboxylic Acid Transport, Regulation of long-term Neuronal Synaptic Plasticity