CXCL5 antibody (Biotin)

Catalog No. ABIN115995

Product Details anti-CXCL5 Antibody (Biotin)

Target: CXCL5 (Chemokine (C-X-C Motif) Ligand 5 (CXCL5))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CXCL5 antibody is conjugated to Biotin

Application: Western Blotting (WB), Enzyme Immunoassay (EIA)

Specificity: This antibody reacts with human Epithelial Neutrophil Activity Protein-78 (hENA-78)

Purification: Affinity chromatography.

Immunogen: Highly pure (>98%) recombinant hENA-78 (human ENA-78).

Application Details

Application Notes: ELISA: (Direct): To detect hENA-78 by direct ELISA (using 100 μL/well antibody solution) aconcentration of 0.25-1.0 μg/mL of this antibody is required. This biotinylated polyclonalantibody, in conjunction with compatible secondary reagents, allows the detection of atleast 0.2-0.4 ng/well of recombinant hENA-78. (Sandwich): To detect hENA-78 by sandwich ELISA (using 100 μL/well antibody solution) aconcentration of 0.25-1.0 μg/mL of this antibody is required. This biotinylated polyclonalantibody, in conjunction with Polyclonal Anti-Human ENA-78 (PP1003P) as a captureantibody, allows the detection of at least 0.2-0.4 ng/well of recombinant hENA-78. Western Blot: To detect hENA-78 by Western Blot analysis this antibody can be used at aconcentration of 0.1-0.2 μg/mL. Used in conjunction with compatible secondary reagentsthe detection limit for recombinant hENA-78 is 1.5-3.0 ng/lane, under either reducing ornon-reducing conditions.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Reconstitution: Restore in sterile PBS containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL.

Buffer: PBS, pH 7.2 without preservatives.

Preservative: Without preservative

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody prior to reconstitution at -20 °C. Following reconstitution the antibody can be stored at 2-8 °C for one month or at -20 °C for longer.

Target Details for CXCL5

Target: CXCL5 (Chemokine (C-X-C Motif) Ligand 5 (CXCL5))

Alternative Name: CXCL5 / ENA78 (CXCL5 Products)

Synonyms: ENA-78 antibody, SCYB5 antibody, Cxcl6 antibody, CXCL5 antibody, GCP-2 antibody, GCP2 antibody, SCYB6 antibody, AMCF-II antibody, LIX antibody, Scyb5 antibody, Scyb6 antibody, C-X-C motif chemokine ligand 5 antibody, C-X-C motif chemokine ligand 6 antibody, chemokine (C-X-C motif) ligand 5 antibody, C-X-C motif chemokine 5 antibody, CXCL5 antibody, Cxcl6 antibody, LOC701946 antibody, LOC703222 antibody, Cxcl5 antibody

Background: CXCL5 is a a 9.9kD inflammatory chemokine that belongs to the CXC chemokine family. CXCL5 is expressed by fibroblasts, is induced by bacterial lipopolysaccharides, and is chemotactic for neutrophils. This chemokine is produced concomitantly with Interleukin 8 (IL8) in response to stimulation with either Interleukin 1 (IL1) or Tumor Necrosis Factor alpha (TNFA). This chemokine is a potent chemotaxin involved in neutrophil activation.Synonyms: C-X-C motif chemokine 5, Cytokine LIX, ENA-78, Epithelial-derived neutrophil-activating protein 78, Neutrophil-activating peptide ENA-78, SCYB5, Small-inducible cytokine B5

Gene ID: 6374

NCBI Accession: NP_002985

UniProt: P42830

Pathways: Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity