CX3CL1 antibody (Biotin)
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- Target See all CX3CL1 Antibodies
- CX3CL1 (Chemokine (C-X3-C Motif) Ligand 1 (CX3CL1))
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Reactivity
- Human
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Host
- Rabbit
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Clonality
- Polyclonal
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Conjugate
- This CX3CL1 antibody is conjugated to Biotin
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Application
- Western Blotting (WB), Enzyme Immunoassay (EIA)
- Specificity
- Human Fractalkine.
- Purification
- Affinity chromatography
- Immunogen
- Highly pure (> 98%) recombinant hFractalkine (human Fractalkine)
- Top Product
- Discover our top product CX3CL1 Primary Antibody
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- Application Notes
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ELISA: (Direct): To detect hFractalkine by direct ELISA (using 100 μL/well antibody solution) aconcentration of 0.25-1.0 μg/mL of this antibody is required. This biotinylated polyclonalantibody, in conjunction with compatible secondary reagents, allows the detection of atleast 0.2-0.4 ng/well of recombinant hFractalkine. (Sandwich): To detect hFractalkine by sandwich ELISA (using 100 μL/well antibodysolution) a concentration of 0.25-1.0 μg/mL of this antibody is required. This biotinylatedpolyclonal antibody, in conjunction with Polyclonal Anti-Human Fractalkine (PP1009P) as acapture antibody, allows the detection of at least 0.2-0.4 ng/well of recombinanthFractalkine. Western Blot: To detect hFractalkine by Western Blot analysis this antibody can be used ata concentration of 0.1-0.2 μg/mL. Used in conjunction with compatible secondary reagentsthe detection limit for recombinant hFractalkine is 1.5-3.0 ng/lane, under either reducing ornon-reducing conditions.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. - Restrictions
- For Research Use only
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- Reconstitution
- Centrifuge vial prior to opening. Restore in sterile PBS containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL.
- Buffer
- PBS, pH 7.2, without preservatives
- Preservative
- Without preservative
- Handling Advice
- Avoid repeated freezing and thawing.
- Storage
- 4 °C/-20 °C
- Storage Comment
- The lyophilized antibody is stable at -20 °C for one year from despatch. The reconstituted antibody is stable for two weeks at 2-8 °C. Frozen aliquots are stable for six months when stored at -20 °C.
- Expiry Date
- 12 months
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- Target
- CX3CL1 (Chemokine (C-X3-C Motif) Ligand 1 (CX3CL1))
- Alternative Name
- Fractalkine / CX3CL1 (CX3CL1 Products)
- Synonyms
- ABCD3 antibody, CX3CL1 antibody, DKFZp459K117 antibody, Cx3c antibody, Scyd1 antibody, ABCD-3 antibody, C3Xkine antibody, CXC3 antibody, CXC3C antibody, NTN antibody, NTT antibody, SCYD1 antibody, fractalkine antibody, neurotactin antibody, AB030188 antibody, AI848747 antibody, CX3C antibody, Cxc3 antibody, D8Bwg0439e antibody, ATP binding cassette subfamily D member 3 antibody, C-X3-C motif chemokine ligand 1 antibody, chemokine (C-X3-C motif) ligand 1 antibody, ABCD3 antibody, CX3CL1 antibody, Cx3cl1 antibody, Abcd3 antibody
- Background
- CX3CL1 is a member of the delta chemokine subfamily that contains a unique CX3C cysteine motif near the N-terminal. Unlike other known chemokines, it is a type 1 membrane protein containing a chemokine domain tethered on a long mucin-like stalk. CX3CL1, a leukocyte chemoattractant, is expressed in various tissues including the brain, heart, lung, kidney, skeletal muscle, and testis. The expression is reported to be up-regulated in endothelial cells and microglia by inflammatory signals. CX3CR1, a specific receptor for fractalkine, mediates both leukocyte migration and adhesion.Synonyms: C-X3-C motif chemokine 1, CX3C membrane-anchored chemokine, FKN, NTT, Neurotactin, SCYD1, Small-inducible cytokine D1
- Gene ID
- 6376
- NCBI Accession
- NP_002987
- UniProt
- P78423
- Pathways
- Synaptic Membrane
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