CXCL7 antibody

Catalog No. ABIN116187

Product Details anti-CXCL7 Antibody

Target: CXCL7 (PPBP) (Pro-Platelet Basic Protein (Chemokine (C-X-C Motif) Ligand 7) (PPBP))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CXCL7 antibody is un-conjugated

Application: Western Blotting (WB), Enzyme Immunoassay (EIA), Functional Studies (Func)

Specificity: This antibody reacts with Human Neutrophil Activating Protein-2 (hNAP-2).

Purification: Affinity Chromatography using an immobilized Human NAP-2 matrix

Immunogen: Highly pure (>98%) E.coli derived recombinant Human NAP-2.

Application Details

Application Notes: Neutralization: To yield one-half maximal inhibition [ND50] of the biological activity ofhNAP-2 (50.0 ng/mL), a concentration of 1.75-3.0 μg/mL of this antibody is required. ELISA: To detect hNAP-2 by direct ELISA (using 100 l/well antibody solution) aconcentration of at least 0.5 μg/mL of this antibody is required. This antigen affinitypurified antibody, in conjunction with compatible secondary reagents, allows the detectionof recombinant hNAP-2. Western Blot: To detect hNAP-2 by Western Blot analysis this antibody can be used at aconcentration of 0.1-0.2 μg/mL. Used in conjunction with compatible secondary reagentsthe detection limit for recombinant hNAP-2 is 1.5-3.0 ng/lane, under either reducing ornon-reducing conditions.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Reconstitution: Restore in sterile water to a concentration of 0.1-1.0 mg/mL.

Buffer: PBS, pH 7.2 without preservatives

Preservative: Without preservative

Handling Advice: Avoid repeated freezing and thawing. Centrifuge vial prior to opening!

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody prior to reconstitution at -20 °C. Following reconstitution the antibody can be stored at 2-8 °C for one month or at -20 °C for longer.

Target Details for CXCL7

Target: CXCL7 (PPBP) (Pro-Platelet Basic Protein (Chemokine (C-X-C Motif) Ligand 7) (PPBP))

Alternative Name: NAP2 / PPBP / CXCL7 (PPBP Products)

Synonyms: 2400003M24Rik antibody, AI854500 antibody, CTAP3 antibody, CTAPIII antibody, Cxcl7 antibody, LA-PF4 antibody, LDGF antibody, MDGF antibody, NAP-2 antibody, NAP-2-L1 antibody, Scyb7 antibody, TGB antibody, TGB1 antibody, THBGB1 antibody, b-TG1 antibody, beta-TG antibody, B-TG1 antibody, Beta-TG antibody, CTAP-III antibody, CXCL7 antibody, PBP antibody, SCYB7 antibody, TC1 antibody, TC2 antibody, THBGB antibody, Nap-2 antibody, CTAPIII/NAP-2 antibody, Platelet basic protein antibody, pro-platelet basic protein antibody, scyb7 antibody, Ppbp antibody, PPBP antibody

Background: NAP2 is a platelet-derived growth factor that belongs to the CXC chemokine family. This growth factor is a potent chemoattractant and activator of neutrophils. It has been shown to stimulate various cellular processes including DNA synthesis, mitosis, glycolysis, intracellular cAMP accumulation, prostaglandin E2 secretion, and sythesis of hyaluronic acid and sulfated glycosaminoglycan. It also stimulates the formation and secretion of plasminogen activator by synovial cells.Synonyms: C-X-C motif chemokine 7, CTAP3, Leukocyte-derived growth factor, Macrophage-derived growth factor, SCYB7, Small-inducible cytokine B7, TGB1, THBGB1

Gene ID: 5473

NCBI Accession: NP_002695

UniProt: P02775