Nerve Growth Factor antibody

Catalog No. ABIN116191

Product Details anti-Nerve Growth Factor Antibody

Target: Nerve Growth Factor (NGF)

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Nerve Growth Factor antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Functional Studies (Func), Enzyme Immunoassay (EIA)

Specificity: Specific for Human beta NGF (Nerve Growth Factor). Other species not tested.

Purification: Affinity Chromatography employing immobilized Human beta-NGF matrix

Immunogen: Highly pure (>98%) E.coli derived recombinant Human beta-NGF.

Application Details

Application Notes: Neutralization: To yield one-half maximal inhibition [ND50] of the biological activity ofhuman beta NGF (1 ng/mL), a concentration of 0.05 - 0.08 μg/mL of this antibody isrequired. ELISA: To detect human beta NGF by direct ELISA (using 100l/well antibody solution) aconcentration of at least 0.5g/mL of this antibody is required. This antigen affinitypurified antibody, in conjunction with compatible secondary reagents, allows the detectionof 0.2 - 0.4 ng/well of recombinant human beta NGF. Western Blot: To detect human beta NGF by Western Blot analysis this antibody can beused at a concentration of 0.1 - 0.2 μg/mL. Used in conjunction with compatible secondaryreagents the detection limit for recombinant human beta NGF is 1.5 - 3.0 ng/lane, undereither reducing or non-reducing conditions. Immunohistochemistry on frozen sections.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Reconstitution: Restore in sterile water to a concentration of 0.1-1.0 mg/mL.

Buffer: PBS, pH 7.2

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody prior to reconstitution at -20 °C. Following reconstitution the antibody can be stored at 2-8 °C for one month or at -20 °C for longer.

Target Details for Nerve Growth Factor

Target: Nerve Growth Factor (NGF)

Alternative Name: NGF (NGF Products)

Synonyms: Beta-NGF antibody, HSAN5 antibody, NGFB antibody, Ngfb antibody, ngfb antibody, ns:zft386 antibody, xx:zft386 antibody, zgc:109730 antibody, beta-NGF antibody, hsan5 antibody, beta-ngf antibody, NGF antibody, ngf antibody, nerve growth factor antibody, nerve growth factor b (beta polypeptide) antibody, nerve growth factor S homeolog antibody, NGF antibody, Ngf antibody, ngfb antibody, ngf antibody, ngf.S antibody

Background: Nerve growth factor (NGF) is one of a family of neurotrophins that induce the survival and proliferation of neurons. In cell culture, NGF induces the formation of neurite projections and, in vivo, may stimulate the innervation of tissues. NGF plays a role in the repair, regeneration, and protection of neurons, and as such could serve as a therapeutic agent in neurodegenerative conditions such as Alzheimer's disease. NGF enhances survival, growth, neurotransmitter biosynthesis of sympathetic and sensory neurons, neurotrophic factor, cutaneous innervation, growth, differentiation and survival of B lymphocytes. It also has a possible role in allergy and tissue repair. NGF is found in the hypothalamus, pituitary, thyroid gland, testes, epididymis, vascular smooth muscle cells, fibroblasts, mast cells and eosinophils. NGF is upregulated by glutamate, vitamin D3, IL6, FGF basic, astrocyte specific IL1, TNF alpha, PDGF and TGF beta. It is downregulated by GABAergic neuronal activity, glucocorticoids and Schwann cell-specific TGF beta.Synonyms: Beta-NGF, Beta-nerve growth factor, NGFB

Gene ID: 4803

NCBI Accession: NP_002497

UniProt: P01138

Pathways: Regulation of Cell Size