CXCL1 antibody (Biotin)

Catalog No. ABIN116258

Product Details anti-CXCL1 Antibody (Biotin)

Target: CXCL1 (Chemokine (C-X-C Motif) Ligand 1 (Melanoma Growth Stimulating Activity, Alpha) (CXCL1))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CXCL1 antibody is conjugated to Biotin

Application: Western Blotting (WB), Enzyme Immunoassay (EIA)

Specificity: This anibody reacts with Growth Related Oncoprotein alpha.

Purification: Affinity chromatography.

Immunogen: Highly pure (>98%) recombinant hGRO/MGSA.

Application Details

Application Notes: ELISA: To detect hGRO/MGSA by direct ELISA (using 100 l/well antibody solution) thisantibody can be used at a concentration of 0.15 - 0.30 μg/mL. Used in conjunction withcompatible secondary reagents, allows the detection of at least 0.2 ng/well of recombinanthGRO/MGSA. Western Blot: To detect hGRO/MGSA by Western Blot analysis this antibody can be used ata concentration of 0.1 - 0.2 μg/mL. Used in conjunction with compatible secondaryreagents the detection limit for recombinant hGRO/MGSA is 1.5 - 3.0 ng/lane, under eitherreducing or non-reducing conditions.

Restrictions: For Research Use only

Handling

Reconstitution: Restore in sterile PBS containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL.

Buffer: PBS, pH 7.2 without preservatives.

Preservative: Without preservative

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody prior to reconstitution at -20 °C. Following reconstitution the antibody can be stored at 2-8 °C for one month or at -20 °C for longer.

Target Details for CXCL1

Target: CXCL1 (Chemokine (C-X-C Motif) Ligand 1 (Melanoma Growth Stimulating Activity, Alpha) (CXCL1))

Alternative Name: CXCL1 / GRO-alpha (CXCL1 Products)

Synonyms: FSP antibody, GRO1 antibody, GROa antibody, MGSA antibody, MGSA-a antibody, NAP-3 antibody, SCYB1 antibody, CINC-1 antibody, Gro1 antibody, CXCL1 antibody, Sdf1 antibody, Fsp antibody, KC antibody, Mgsa antibody, N51 antibody, Scyb1 antibody, gro antibody, GRO antibody, Gro antibody, C-X-C motif chemokine ligand 1 antibody, chemokine (C-X-C motif) ligand 1 (melanoma growth stimulating activity, alpha) antibody, C-X-C motif chemokine ligand 12 antibody, chemokine (C-X-C motif) ligand 1 antibody, CXCL1 antibody, Cxcl1 antibody, Cxcl12 antibody, GRO1 antibody

Background: The GRO gene was originally identified by subtractive hybridization studies between normal and tumorigenic Chinese hamster embryo fibroblasts. The hamster cDNA was cloned and used as a probe for cloning of the human GRO cDNA. The GROalpha gene initially cloned from T24 cells and the gene in melanoma cells encoding melanoma growth stimulating protein (MGSA) are identical. Human cells contain three closely related, but distinct GRO genes: GRO alpha, GRO beta, and GRO gamma. GRO beta and GRO gamma share 93 % and 82 % identity, respectively, with GRO alpha at the nucleotide level. GROs are members of the chemokine alpha family that is characterized by the separation with one amino acid of the first two cysteine residues, C-X-C, in the amino acid sequence. The GRO gene has been mapped to chromosome 4q21. In normal cells, human mRNA GRO expression is found in foreskin fibroblasts, synovial fibroblasts, chondrocytes and osteocytes. Additionally, GRO mRNA has been detected in mammary fibroblasts, mammary epithelial cells, endothelial cells, activated monocytes, macrophages, and neutrophils. Characterization of the GROalpha receptor indicates the presence of low and high affinity receptors on human neutrophils.Synonyms: CXCL1, GRO, GRO-alpha(1-73), GRO1, GROA, Growth-regulated alpha protein, MGSA, Melanoma growth stimulatory activity, NAP-3, Neutrophil-activating protein 3, SCYB1

Gene ID: 2919

NCBI Accession: NP_001502

UniProt: P09341

Pathways: Autophagy