CLEC11A antibody (Biotin)

Catalog No. ABIN116315

Product Details anti-CLEC11A Antibody (Biotin)

Target: CLEC11A (C-Type Lectin Domain Family 11, Member A (CLEC11A))

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This CLEC11A antibody is conjugated to Biotin

Application: Western Blotting (WB), Enzyme Immunoassay (EIA)

Specificity: Stem Cell Growth Factor beta (SCGF-beta)

Cross-Reactivity (Details): Species reactivity (tested):Human.

Purification: Affinity Chromatography.

Immunogen: Highly pure (>98%) recombinant human SCGF-beta.

Application Details

Application Notes: Direct ELISA: To detect Human SCGF-Beta by Direct ELISA (using 100 μL/well) aconcentration of approx. 1.0 μg/mL of this antibody is required. This Biotinylated antibody allowsthe detection of at least 0.2-0.4 ng/well of recombinant Human SCGF-Beta. Sandwich ELISA: To detect Human SCGF-Beta by Sandwich ELISA (using 100 μL/well) thisantibody can be used at a concentration of 0.25-1.0 μg/mL. Used in conjunction withpurified anti-Human SCGF beta antibody (PP1106P1 or PP1106P2) as a Capture antibody,allows the detection of at least 0.2-0.4 ng/well of recombinant Human SCGF-Beta. Western Blot: To detect Human SCGF-Beta by Western Blot analysis this antibody can beused at a concentration of 0.1-0.2 μg/mL. Used in conjunction with compatible secondaryreagents the detection limit for recombinant hSCGF-Beta is 1.5-3.0 ng/lane, under eitherreducing or non-reducing conditions.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Reconstitution: Restore in sterile PBS containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL.

Buffer: PBS, pH 7.2 without preservatives.

Preservative: Without preservative

Handling Advice: Avoid repeated freezing and thawing. Centrifuge vial prior to opening!

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody prior to reconstitution at -20 °C. Following reconstitution the antibody can be stored at 2-8 °C for one month or at -20 °C for longer.

Target Details for CLEC11A

Target: CLEC11A (C-Type Lectin Domain Family 11, Member A (CLEC11A))

Alternative Name: SCGF / CLEC11A (CLEC11A Products)

Synonyms: CLEC11A antibody, CLECSF3 antibody, LSLCL antibody, P47 antibody, SCGF antibody, AW457320 antibody, Clecsf3 antibody, Scgf antibody, C-type lectin domain containing 11A antibody, C-type lectin domain family 11, member a antibody, CLEC11A antibody, Clec11a antibody

Background: This cytokine stimulates the proliferation and differentiation of hematopoietic precursor cells from various lineages, including erythrocytes, lymphocytes, granulocytes and macrophages. It acts synergistically with other cytokines, including IL3, GCSF, GMCSF and FLT3 ligand and suppresses SCF-stimulated erythrocyte proliferation.Synonyms: C-type lectin domain family 11 member A, C-type lectin superfamily member 3, CLECSF3, LSLCL, Lymphocyte secreted C-type lectin, Stem cell growth factor, p47

Gene ID: 6320

NCBI Accession: NP_002966

UniProt: Q9Y240