IL1A antibody

Catalog No. ABIN116387

Product Details anti-IL1A Antibody

Target: IL1A (Interleukin 1 alpha (IL1A))

Reactivity: Rat

Host: Goat

Clonality: Polyclonal

Conjugate: This IL1A antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA), Functional Studies (Func)

Specificity: This antibody reacts with Rat Interleukin 1 alpha

Purification: Affinity chromatography

Immunogen: Recombinant rat IL-1 alpha

Application Details

Application Notes: Immunohistochemistry: we recommend use of 1 μg per ml as a starting dilution. Paraffin sections may require pretreatment like Proteinase K or Trypsin predigestion toimprove staining. Neutralization: To yield one-half maximal inhibition [ND50] of the biological activity of rIL-1alpha (50 pg/mL), a concentration of 0.011-0.017 μg/mL of this antibody is required. ELISA: To detect rIL-1 alpha by direct ELISA (using 100 ml/well antibody solution) aconcentration of at least 0.5 μg/mL of this antibody is required. This antigen affinitypurified antibody, in conjunction with compatible secondary reagents, allows the detectionof 0.2-0.4 ng/well of recombinant rIL-1 alpha. Western blot: To detect rIL-1 alpha by Western Blot analysis this antibody can be used at aconcentration of 0.1-0.2 μg/mL. Used in conjunction with compatible secondary reagentsthe detection limit for recombinant rIL-1 alpha is 1.5-3.0 ng/lane, under either reducing ornon-reducing conditions.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Reconstitution: Restore in sterile water to a concentration of 0.1-1.0 mg/mL.

Buffer: PBS, pH 7.2 without preservatives.

Preservative: Without preservative

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody prior to reconstitution at -20 °C. Following reconstitution the antibody can be stored at 2-8 °C for one month or at -20 °C for longer.

Target Details for IL1A

Target: IL1A (Interleukin 1 alpha (IL1A))

Alternative Name: Interleukin-1 alpha / IL-1A (IL1A Products)

Synonyms: IL-1A antibody, IL1 antibody, IL1-ALPHA antibody, IL1F1 antibody, IL-6 antibody, Il-1a antibody, IL-1 alpha antibody, IL-1alpha antibody, L1A antibody, interleukin 1 alpha antibody, interleukin 6 antibody, IL1A antibody, IL6 antibody, Il1a antibody

Background: Interleukins (ILs) are a large group of cytokines that are produced mainly by leukocytes, although some are produced by certain phagocytes and auxiliary cells. Each IL acts on a specific, limited group of cells through a receptor specific for that IL. Interleukin 1 (IL1), originally known as lymphocyte activating factor (LAF), activates T cells and lymphocytes, which then proliferate and secrete interleukin 2. IL1 is primarily released from stimulated macrophages and monocytes, but also is released from several other cell types and is thought to play a key role in inflammatory and immune responses. The two closely related agents, interleukin 1 alpha (IL1 alpha) and interleukin 1 beta (IL1 beta) bind to the same cell surface receptor, elicit nearly identical biological responses and share 25 % homology in their amino acid sequence.Synonyms: Hematopoietin-1, IL-1 alpha, IL1A, IL1F1

Gene ID: 24493

NCBI Accession: NP_058715

UniProt: P16598

Pathways: NF-kappaB Signaling, Autophagy, Cancer Immune Checkpoints