GDF3
Reactivity: Human, Mouse, Rat
WB, IHC, ELISA, ICC, IF
Host: Rabbit
Polyclonal
unconjugated
Application Notes
ELISA: To detect hGDF-3 by direct ELISA (using 100 l/well antibody solution) this antibodycan be used at a concentration of 0.15 - 0.30 μg/mL. Used in conjunction with compatiblesecondary reagents, allows the detection of at least 0.2 ng/well of recombinant hGDF-3. Western blot: To detect hGDF-3 by Western Blot analysis this antibody can be used at aconcentration of 0.1 - 0.2 μg/mL. Used in conjunction with compatible secondary reagentsthe detection limit for recombinant hGDF-3 is 1.5 -3.0 ng/lane, under either reducing ornon-reducing conditions. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions
For Research Use only
Reconstitution
Restore to a concentration of 50 μg/mL with sterile PBS solution containing 0.1 % BSA.
Buffer
BPS without preservatives.
Preservative
Without preservative
Handling Advice
Avoid repeated freezing and thawing.
Storage
4 °C
Storage Comment
Store the antibody at 2-8 °C for short term only. After reconstitution store and aliquot at -20 °C for longer.
GDF3 is a member of the bone morphogenetic protein (BMP) family and the TGF-beta superfamily. This group of proteins is characterized by a polybasic proteolytic processing site which is cleaved to produce a mature protein containing seven conserved cysteine residues. The members of this family are regulators of cell growth and differentiation in both embryonic and adult tissues. The function of this protein is unknown, but expression studies suggest it may be involved in regulation of the adult lymphatic and erythroid systems and embryonic development.Synonyms: GDF-3, Growth/differentiation factor 3