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DEFB103A antibody (Biotin)

DEFB103A Reactivity: Human WB, EIA Host: Rabbit Polyclonal Biotin
Catalog No. ABIN116503
  • Target See all DEFB103A Antibodies
    DEFB103A (Defensin, beta 103A (DEFB103A))
    Reactivity
    • 8
    • 1
    Human
    Host
    • 4
    • 3
    • 1
    Rabbit
    Clonality
    • 6
    • 2
    Polyclonal
    Conjugate
    • 8
    This DEFB103A antibody is conjugated to Biotin
    Application
    • 7
    • 3
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    Western Blotting (WB), Enzyme Immunoassay (EIA)
    Specificity
    This antibody reacts with Human BD3 (Defensin beta 3). Other species not tested.
    Purification
    Affinity Chromatography
    Immunogen
    E.coli derived recombinant Human Beta Defensin-3.
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    Discover our top product DEFB103A Primary Antibody
  • Application Notes
    ELISA: Direct: To detect Human BD-3 by direct ELISA (using 100 μL/well antibody solution) thisantibody can be used at a concentration of 1.0 μg/mL. Used in conjunction with compatiblesecondary reagents, allows the detection of at least 0.2-0.4 ng/well of recombinant HumanBD-3. Sandwich: To detect Human BD-3 by Sandwich ELISA (using 100 μL/well antibody solution)this antibody can be used at a concentration of 0.25-1.0 μg/mL. This biotinylated antibody,used in conjunction with polyclonal anti-human BD-3, allows the detection of at least0.2-0.4 ng/well of recombinant Human BD-3. Western blot: To detect Human BD-3 by Western Blot analysis this antibody can be used ata concentration of 0.1-0.2 μg/mL. Used in conjunction with compatible secondary reagentsthe detection limit for recombinant hBD-3 is 1.5-3.0 ng/lane, under either reducing ornon-reducing conditions.
    Other applications not tested.
    Optimal dilutions are dependent on conditions and should be determined by the user.
    Restrictions
    For Research Use only
  • Reconstitution
    Centrifuge vial prior to opening. Restore in sterile PBS containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL
    Buffer
    BPS, pH 7.2 without preservatives
    Preservative
    Without preservative
    Handling Advice
    Avoid repeated freezing and thawing.
    Storage
    4 °C/-20 °C
    Storage Comment
    Prior to reconstitution store at 2-8 °C. Following reconstitution store the antibody at -20 °C.
  • Target
    DEFB103A (Defensin, beta 103A (DEFB103A))
    Alternative Name
    Defensin beta 3 (DEFB103A Products)
    Synonyms
    BD-3 antibody, DEFB-3 antibody, DEFB103 antibody, DEFB3 antibody, HBD3 antibody, HBP-3 antibody, HBP3 antibody, hBD-3 antibody, DEFB103B antibody, defensin beta 103A antibody, defensin beta 103B antibody, defensin, beta 103A antibody, beta-defensin 103 antibody, DEFB103A antibody, DEFB103B antibody, LOC694235 antibody, LOC100359096 antibody
    Background
    Beta Defensin 3 exhibits antimicrobial activity against Gram-positive bacteria S.aureus and S.pyogenes, Gram-negative bacteria P.aeruginosa and E.coli and the yeast C.albicans. It kills multiresistant S.aureus and vancomycin-resistant E.faecium, but it appears to have no significant hemolytic activity. Beta Defensin 3 is highly expressed in skin and tonsils, and to a lesser extent in trachea, uterus, kidney, thymus, adenoid, pharynx and tongue. Low expression is observed in salivary gland, bone marrow, colon, stomach, polyp and larynx, with no expression in small intestine. Expression is induced by infection of bacteria and by interferon gamma. Beta Defensin 3 belongs to the beta-defensin family.Synonyms: BD3, Beta-defensin 103, DEFB103, DEFB103A, DEFB103B, DEFB3, Defensin beta 103, Defensin-like protein, HBD3, HBP3
    Gene ID
    414325
    NCBI Accession
    NP_001075020
    UniProt
    P81534
    Pathways
    Production of Molecular Mediator of Immune Response
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