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Cyclin E1 antibody (CCNE1)

Details for Product anti-CCNE1 Antibody No. ABIN116512, Supplier: Log in to see
Antigen
  • AW538188
  • Ccne
  • CCNE
  • ccne
  • CCNE1
  • cyce
  • CycE1
  • cyce2
  • cycE3
  • CYCLE
  • cyclinE
  • fa18e07
  • wu:fa18e07
Alternatives
anti-Human Cyclin E1 antibody for ELISA
Reactivity
Human, Mouse (Murine), Rat (Rattus)
251
51
44
7
3
3
3
2
2
Host
Rabbit
227
46
2
Clonality
Polyclonal
Conjugate
This Cyclin E1 antibody is un-conjugated
7
4
4
3
2
2
2
2
2
2
2
2
2
2
2
1
Application
Enzyme Immunoassay (EIA), Immunoprecipitation (IP), Western Blotting (WB)
182
97
73
70
59
33
31
23
15
6
5
3
3
3
2
2
1
1
1
Supplier
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Immunogen Full length synthetic polypeptide corresponding to the human gene sequence.
Specificity This product was prepared from monospecific antiserum by delipidation and defibrination. Antiserum will specifically react with a 45 kDa Cyclin E protein. No reaction was observed against other related cyclins. Cross reactivity with Cyclin E from other species may also occur.
Purification Serum
Alternative Name Cyclin E1 (CCNE1 Antibody Abstract)
Background Cyclin E is a regulatory subunit of Cdk2 and controls G1 / S transition during the mammalian cell cycle. Multiple isoforms of Cyclin E are only expressed in tumors but not in normal tissue, suggesting a post transcriptional regulation of Cyclin E. In vitro analyses indicated that these truncated variant isoforms of Cyclin E are able to phosphorylate histone H1. Alterations in the Cyclin E protein have been implicated as indicators of worse prognosis in various cancers.Synonyms: CCNE, CCNE1, Cyclin-E1
Gene ID 898
NCBI Accession NP_001229
UniProt P24864
Research Area Cell Cycle
Pathways Cell Division Cycle
Application Notes ELISA: 1: 2,000. Immunoprecipitation. Western Blot: 1: 200 to 1: 500.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Format Liquid
Concentration 85 mg/mL (by Refractometry)
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store the antibody at 2 - 8 °C up to one month or (in aliquots) at -20 °C for longer.
Background publications Claudio, De Luca, Howard, Baldi, Firpo, Koff, Paggi, Giordano: "Functional analysis of pRb2/p130 interaction with cyclins." in: Cancer research, Vol. 56, Issue 9, pp. 2003-8, 1996

Koff, Cross, Fisher, Schumacher, Leguellec, Philippe, Roberts: "Human cyclin E, a new cyclin that interacts with two members of the CDC2 gene family." in: Cell, Vol. 66, Issue 6, pp. 1217-28, 1991