Collagen Type I antibody

Catalog No. ABIN116549

Product Details anti-Collagen Type I Antibody

Target: Collagen Type I (COL1) (Collagen, Type I (COL1))

Reactivity: Human, Rat, Mouse, Cow, Mammalian

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Collagen Type I antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Enzyme Immunoassay (EIA)

Specificity: This product has been prepared by Immunoaffinity Chromatography using immobilized antigens followed by extensive cross-adsorption against other collagens, human serum proteins and non-collagen extracellular matrix proteins to remove any unwanted specificities. Typically less than 1 % cross reactivity against other types of collagens was detected by ELISA against purified standards. Some class-specific anti-collagens may be specific for three-dimensional epitopes which may result in diminished reactivity with denatured collagen or formalin-fixed, paraffin embedded tissues. This antibody reacts with most mammalian Type I Collagens and has negligible cross-reactivity with Type II, III, IV, V or VI collagens. Non-specific cross-reaction of anti-collagen antibodies with other Human serum proteins or non-collagen extracellular matrix proteins is negligible.

Purification: Immunoaffinity Chromatography.

Immunogen: Collagen type I purified from Human and Bovine placenta.

Application Details

Application Notes: Anti-Collagen antibodies have been used for indirect trapping ELISA for quantitation ofantigen in serum using a standard curve, for Immunoprecipitation and for native(non-denaturing, non-dissociating) PAGE and western blotting for highly sensitivequalitative analysis. Specific researchers have reported that this antibody is alsofunctional by conventional SDS-PAGE western blot. See references below for additionaldetails. Recommended Dilutions: ELISA: 1/5,000-1/50,000. Western blot: 1/500-1/5,000. Immunoprecipitation: 1/100. Immunohistochemistry: 1/50-1/200 (prepare tissues as frozen tissue sections).

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL (by UV absorbance at 280 nm)

Buffer: 0.125 M Sodium Borate, 0.075 M Sodium Chloride, 0.005 M EDTA, pH 8.0, 0.01 % (w/v) Sodium Azide, None

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing. This product is photosensitive and should be protected from light.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

References for anti-Collagen Type I antibody (ABIN116549)

Yamanaka, Menuki, Tajima, Okada, Kosugi, Zenke, Sakai: "Effect of estradiol on fibroblasts from postmenopausal idiopathic carpal tunnel syndrome patients." in: Journal of cellular physiology, Vol. 233, Issue 11, pp. 8723-8730, (2018) (PubMed).

Caley, King, Shah, Wang, Rodriguez-Teja, Gronau, Waxman, Sturge: "Tumor-associated Endo180 requires stromal-derived LOX to promote metastatic prostate cancer cell migration on human ECM surfaces." in: Clinical & experimental metastasis, Vol. 33, Issue 2, pp. 151-65, (2016) (PubMed).

González-Miguel, Morchón, Siles-Lucas, Simón: "Fibrinolysis and proliferative endarteritis: two related processes in chronic infections? The model of the blood-borne pathogen Dirofilaria immitis." in: PLoS ONE, Vol. 10, Issue 4, pp. e0124445, (2015) (PubMed).

Carriel, Scionti, Campos, Roda, Castro, Cornelissen, Garzón, Alaminos: "In vitro characterization of a nanostructured fibrin agarose bio-artificial nerve substitute." in: Journal of tissue engineering and regenerative medicine, Vol. 11, Issue 5, pp. 1412-1426, (2015) (PubMed).

Nyström, Thriene, Mittapalli, Kern, Kiritsi, Dengjel, Bruckner-Tuderman: "Losartan ameliorates dystrophic epidermolysis bullosa and uncovers new disease mechanisms." in: EMBO molecular medicine, Vol. 7, Issue 9, pp. 1211-28, (2015) (PubMed).

Behrens, Villone, Koch, Brunner, Sorokin, Robenek, Bruckner-Tuderman, Bruckner, Hansen: "The epidermal basement membrane is a composite of separate laminin- or collagen IV-containing networks connected by aggregated perlecan, but not by nidogens." in: The Journal of biological chemistry, Vol. 287, Issue 22, pp. 18700-9, (2012) (PubMed).

Villone, Fritsch, Koch, Bruckner-Tuderman, Hansen, Bruckner: "Supramolecular interactions in the dermo-epidermal junction zone: anchoring fibril-collagen VII tightly binds to banded collagen fibrils." in: The Journal of biological chemistry, Vol. 283, Issue 36, pp. 24506-13, (2008) (PubMed).

Target Details for Collagen Type I

Target: Collagen Type I (COL1) (Collagen, Type I (COL1))

Alternative Name: Collagen Type I (COL1 Products)

Synonyms: collagen type III alpha 1 chain antibody, COL3A1 antibody

Background: Collagens are highly conserved throughout evolution and are characterized by an uninterrupted "Glycine-X-Y" triplet repeat that is a necessary part of the triple helical structure. For these reasons, it is often extremely difficult to generate antibodies with specificities to collagens. The development of type specific antibodies is dependent on NON-DENATURED three-dimensional epitopes. Collagens for immunization from human and bovine placenta and cartilage have been extensively purified by limited pepsin digestion and selective salt precipitation. This preparation results in a native conformation of the protein. Antibodies are isolated from rabbit antiserum and are extensively cross-adsorbed by immunoaffinity purification to produce 'type' specific antibodies. Greatly diminished reactivity and selectivity of these antibodies will result if denaturing and reducing conditions are used for SDS PAGE and immunoblotting.Synonyms: Alpha-1 type I collagen, Alpha-2 type I collagen, COL1A1, COL1A2

Gene ID: 1277, 9606

UniProt: P02452