Assay by immunoelectrophoresis resulted in a single precipitin arc against purified and partially purified Alpha-1-Acid Glycoprotein [Human Plasma]. Cross reactivity against Alpha-1-Acid Glycoprotein from other tissues and species may occur but have not been specifically determined.
Purification
Prepared from monospecific antiserum by a delipidation and defibrination.
Suitable for Immunoblotting (Western or Dot blot), ELISA, Immunoprecipitation and mostimmunological methods requiring high titer and specificity. Recommended Dilutions: This product has been assayed against 1.0 μg of Alpha-1-AcidGlycoprotein [Human Plasma] in a standard sandwich ELISA using Peroxidase conjugatedAffinity Purified anti-Rabbit IgG [H&L] (Goat) and ABTS(2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes atroom temperature. A working dilution of 1: 2,000 to 1: 10,000 of the reconstitutionconcentration is suggested for this product. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions
For Research Use only
Format
Liquid
Reconstitution
Restore with 2.0 mL of deionized water (or equivalent).
Concentration
90.0 mg/mL (by Refractometry)
Buffer
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 with 0.01 % sodium azide as preservative.
Preservative
Sodium azide
Precaution of Use
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice
Dilute only prior to immediate use. Avoid cycles of freezing and thawing.
Storage
-20 °C
Storage Comment
Store vial at -20 °C prior to opening. Centrifuge product if not completely clear after standing at room temperature. For extended storage aliquot contents and freeze at -20 °C or below.
Alpha 1 Acid Glycoprotein (AGP) is an acute phase plasma protein synthesized by the liver. The protein is believed to regulate the interaction between blood cells and endothelial cells, and together with haptoglobin and C reactive protein, also regulates the extravasation of the cells during infection and inflammation. AGP is a major acute phase reactant, both in human and in rat. In human plasma, AGP (44 kDa) is found at levels of 0.5-1.4 mg/mL. Its concentration in blood plasma is elevated during acute inflammation, such as trauma, malignancies, myocardial infarction, rheumatoid arthritis, bacterial infections, after major surgery, in cases of chronic pain etc, and can be used for the diagnosis of inflammatory conditions.Synonyms: Alpha-1-acid glycoprotein 1, OMD1, Orosomucoid-1