Alcohol Dehydrogenase 1A (Class I), alpha Polypeptide (ADH1A) antibody (Biotin)

Antigen: Alcohol Dehydrogenase 1A (Class I), alpha Polypeptide (ADH1A)

Synonyms: ADH1, Adh-1, ADH-AA, Adh-1e, Adh-1t, Adh-3e, Adh1-e, Adh1-t, Adh1tl, Adh3-e, Adh-1-t, AI194826, Adh, Adh1a, ADH, ALCOHOL DEHYDROGENASE, ALCOHOL DEHYDROGENASE 1, ARABIDOPSIS THALIANA ALCOHOL DEHYDROGENASE, ATADH, ATADH1, F22K20.19, F22K20_19, ADC1, DvirAdh, dvir_GLEANR_2771, DvirGJ18208, GJ18208, Adh1, DKFZp470F1127

Clonality: Polyclonal

Host: Rabbit

Reactivity: Yeast (Pichia pastoris)

Conjugate: Biotin

Application: Enzyme Immunoassay (EIA), Western Blotting (WB)

Catalog no.: ABIN116589

Additional Information

Characteristics: Synonyms: Alcohol dehydrogenase I, YADH-1, ADH1, ADC1, YOL086C, O0947

Alternative name: Alcohol dehydrogenase 1

Swiss-Prot: P00330

Immunogen: Alcohol Dehydrogenase [Yeast]

Specificity: Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Biotin,anti-Rabbit Serum as well as purified and partially purified Alcohol Dehydrogenase [Yeast]. Cross reactivity against Alcohol Dehydrogenase from other sources may occur but have notbeen specifically determined.

Synonyms: ADH1, Adh-1, ADH-AA, Adh-1e, Adh-1t, Adh-3e, Adh1-e, Adh1-t, Adh1tl, Adh3-e, Adh-1-t, AI194826, Adh, Adh1a, ADH, ALCOHOL DEHYDROGENASE, ALCOHOL DEHYDROGENASE 1, ARABIDOPSIS THALIANA ALCOHOL DEHYDROGENASE, ATADH, ATADH1, F22K20.19, F22K20_19, ADC1, DvirAdh, dvir_GLEANR_2771, DvirGJ18208, GJ18208, Adh1, DKFZp470F1127

Application Details

Application Notes: Immunoblotting, ELISA, immunohistochemistry, immunomicroscopy as well as otherantibody based assays using streptavidin or avidin conjugates requiring lot-to-lotconsistency. This product has been assayed against 1.0 ug of Alcohol Dehydrogenase in a standardcapture ELISA using Peroxidase Conjugated Streptavidin and ABTS(2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes atroom temperature. A working dilution of 1: 4,000 to 1: 20,000 of the reconstitution concentration is suggestedfor this product. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.

Concentration: 10.0 mg/ml (by UV absorbance at 280 nm)

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2,containing 10 mg/ml BSA (IgG and Protease free) and 0.01% (w/v) Sodium AzideLabel: Biotinamidocaproate N-Hydroxysuccinimide Ester (BAC) Molar Ratio: 10-20 BAC molecules per Rabbit IgG moleculeRec

Storage: Store vial at 2-8°C prior to restoration. Restore with deionized water (or equivalent), centrifuge product if not completely clearafter standing at room temperature. This product is stable for one month at 2-8°C as anundiluted liquid. For extended storage reconstitute product with 50% glycerol instead of water and thenaliquot contents and freeze at -20°C or below. Avoid repeated freezing and thawing. Dilute only prior to immediate use. Shelf life: one year from despatch.

Research Area: Enzymes

Restrictions: For Research Use only

Publications

Publications: Bayer, Wilchek: "Protein biotinylation." in: Methods in enzymology, Vol. 184, pp. 138-60, 1990 (PubMed).