Alcohol Dehydrogenase 1A (Class I), alpha Polypeptide (ADH1A) antibody (HRP)

Antigen: Alcohol Dehydrogenase 1A (Class I), alpha Polypeptide (ADH1A)

Synonyms: ADH1, Adh-1, ADH-AA, Adh-1e, Adh-1t, Adh-3e, Adh1-e, Adh1-t, Adh1tl, Adh3-e, Adh-1-t, AI194826, Adh, Adh1a, ADH, ALCOHOL DEHYDROGENASE, ALCOHOL DEHYDROGENASE 1, ARABIDOPSIS THALIANA ALCOHOL DEHYDROGENASE, ATADH, ATADH1, F22K20.19, F22K20_19, ADC1, DvirAdh, dvir_GLEANR_2771, DvirGJ18208, GJ18208, Adh1, DKFZp470F1127

Clonality: Polyclonal

Host: Rabbit

Reactivity: Yeast (Pichia pastoris)

Conjugate: HRP

Application: Enzyme Immunoassay (EIA), Western Blotting (WB)

Catalog no.: ABIN116590

Additional Information

Characteristics: Synonyms: Alcohol dehydrogenase I, YADH-1, ADH1, ADC1, YOL086C, O0947

Alternative name: Alcohol dehydrogenase 1

Swiss-Prot: P00330

Immunogen: Alcohol Dehydrogenase [Yeast].

Description: ADH (alcohol dehydrogenase) family of proteins metabolize a variety of substances suchas ethanol, retinal, other aliphatic alcohol, hydroxysteroids, and lipid peroxidationproducts. With the coenzyme NAD, ADH catalyzes the reversible conversion of organicalcohol to ketones or aldehydes. ADH play a major role in ethanol metabolism.

Specificity: This product is an IgG fraction antibody purified from monospecific antiserum by amulti-step process which includes delipidation, salt fractionation and ion exchangechromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc againstanti-Peroxidase, anti-Rabbit Serum as well as purified and partially purified AlcoholDehydrogenase [Yeast]. Cross reactivity against Alcohol Dehydrogenase from other sources is unknown.

Synonyms: ADH1, Adh-1, ADH-AA, Adh-1e, Adh-1t, Adh-3e, Adh1-e, Adh1-t, Adh1tl, Adh3-e, Adh-1-t, AI194826, Adh, Adh1a, ADH, ALCOHOL DEHYDROGENASE, ALCOHOL DEHYDROGENASE 1, ARABIDOPSIS THALIANA ALCOHOL DEHYDROGENASE, ATADH, ATADH1, F22K20.19, F22K20_19, ADC1, DvirAdh, dvir_GLEANR_2771, DvirGJ18208, GJ18208, Adh1, DKFZp470F1127

Application Details

Application Notes: Suitable for Immunoblotting (Western or dot blot), ELISA, Immunoperoxidase electronmicroscopy and Immunohistochemistry as well as other peroxidase-antibody basedenzymatic assays. Recommended Dilutions: ELISA 1/4,000-1/20,000. Western blot: 1/1,000-1/5,000. Immunohistochemistry: 1/500-1/2,500. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.

Concentration: 10.0 mg/ml (by UV absorbance at 280 nm)

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 with 0.01%Gentamicin Sulfate as preservative and 10 mg/ml Bovine Serum Albumin (BSA, IgG andProtease free) as stabilizer.Label: Horseradish Peroxidase (HRP).Reconstitution: Restore with 2.0 ml of

Storage: Store vial at 2-8°C prior to restoration. Centrifuge product if not completely clear afterstanding at room temperature. For extended storage reconstitute product with 50%glycerol instead of water and then aliquot contents and freeze at -20°C or below. This product is stable for one month at 2-8°C as an undiluted liquid. Dilute only prior to immediate use. Avoid cycles of freezing and thawing. Shelf life: One year from despatch. Caution: Do Not Use Sodium azide as Preservative.

Research Area: Enzymes

Restrictions: For Research Use only

Publications

Publications: Farr, Nakane: "Immunohistochemistry with enzyme labeled antibodies: a brief review." in: Journal of immunological methods, Vol. 47, Issue 2, pp. 129-44, 1982 (PubMed).