Alcohol Dehydrogenase 1A (Class I), alpha Polypeptide (ADH1A) antibody

Antigen: Alcohol Dehydrogenase 1A (Class I), alpha Polypeptide (ADH1A)

Synonyms: ADH1, Adh-1, ADH-AA, Adh-1e, Adh-1t, Adh-3e, Adh1-e, Adh1-t, Adh1tl, Adh3-e, Adh-1-t, AI194826, Adh, Adh1a, ADH, ALCOHOL DEHYDROGENASE, ALCOHOL DEHYDROGENASE 1, ARABIDOPSIS THALIANA ALCOHOL DEHYDROGENASE, ATADH, ATADH1, F22K20.19, F22K20_19, ADC1, DvirAdh, dvir_GLEANR_2771, DvirGJ18208, GJ18208, Adh1, DKFZp470F1127

Clonality: Polyclonal

Host: Rabbit

Reactivity: Yeast (Pichia pastoris)

Application: Enzyme Immunoassay (EIA), Western Blotting (WB)

Catalog no.: ABIN116591

Additional Information

Characteristics: Synonyms: Alcohol dehydrogenase I, YADH-1, ADH1, ADC1, YOL086C, O0947

Alternative name: Alcohol dehydrogenase 1

Swiss-Prot: P00330

Immunogen: Alcohol Dehydrogenase [Yeast].

Specificity: This product is an IgG fraction antibody purified from monospecific antiserum by amulti-step process which includes delipidation, salt fractionation and ion exchangechromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-RabbitSerum as well as purified and partially purified Alcohol Dehydrogenase [Yeast]. Cross reactivity against Alcohol Dehydrogenase from other sources may occur but have notbeen specifically determined.

Synonyms: ADH1, Adh-1, ADH-AA, Adh-1e, Adh-1t, Adh-3e, Adh1-e, Adh1-t, Adh1tl, Adh3-e, Adh-1-t, AI194826, Adh, Adh1a, ADH, ALCOHOL DEHYDROGENASE, ALCOHOL DEHYDROGENASE 1, ARABIDOPSIS THALIANA ALCOHOL DEHYDROGENASE, ATADH, ATADH1, F22K20.19, F22K20_19, ADC1, DvirAdh, dvir_GLEANR_2771, DvirGJ18208, GJ18208, Adh1, DKFZp470F1127

Application Details

Application Notes: Suitable for Immunoblotting (Western or Dot blot), ELISA, Immunoprecipitation,Conjugation and most immunological methods requiring high titer and specificity. Recommended Dilutions: This product has been assayed against 1.0 m g of AlcoholDehydrogenase [Yeast] in a standard sandwich ELISA using Peroxidase conjugated AffinityPurified anti-Rabbit IgG [H&L] (Goat) and ABTS(2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes atroom temperature. A working dilution of 1: 10,000 to 1: 40,000 of the reconstitutionconcentration is suggested for this product. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.

Concentration: 10.0 mg/ml (by UV absorbance at 280 nm)

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 with 0.01%sodium azide as preservative.Reconstitution: Restore with 5.0 ml of deionized water (or equivalent).

Storage: Store vial at 2-8°C prior to restoration. Centrifuge product if not completely clear afterstanding at room temperature. For extended storage aliquot contents and freeze at -20°Cor below. This product is stable for one month at 2-8°C as an undiluted liquid. Dilute only prior to immediate use. Avoid cycles of freezing and thawing. Shelf life: One year from despatch.

Research Area: Enzymes

Restrictions: For Research Use only