Aldehyde Dehydrogenase (ALDH) (pan) antibody (Biotin)

Antigen: Aldehyde Dehydrogenase (ALDH)

Synonyms: 146084_at, ACDH, ALDH, DmelCG3752, CG3752, Tb06.26G9.1140

Epitope: pan

Clonality: Polyclonal

Host: Rabbit

Reactivity: Yeast (Pichia pastoris)

Conjugate: Biotin

Application: Enzyme Immunoassay (EIA), Western Blotting (WB)

Catalog no.: ABIN116593

Additional Information

Characteristics: Synonyms: ALDH

Alternative name: Aldehyde Dehydrogenase (pan ALDH)

Immunogen: Aldehyde Dehydrogenase [Yeast]

Specificity: This product is an IgG fraction antibody purified from monospecific antiserum by amultistep process which includes delipidation, salt fractionation and ion exchangechromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Biotin,anti-Rabbit Serum as well as purified and partially purified Aldehyde Dehydrogenase[Yeast]. Cross reactivity against Aldehyde Dehydrogenase from other sources may occur but havenot been specifically determined.

Application Details

Application Notes: Suitable for Immunoblotting, ELISA, Immunohistochemistry, Immunomicroscopy as well asother antibody based assays using streptavidin or avidin conjugates requiring lot-to-lotconsistency. Recommended Dilutions: This product has been assayed against 1.0 ug of AldehydeDehydrogenase in a standard capture ELISA using Peroxidase Conjugated Streptavidin andABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutesat room temperature. A working dilution of 1: 1,000 to 1: 5,000 of the reconstitutionconcentration is suggested for this product. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.

Concentration: 10.0 mg/ml (by UV absorbance at 280 nm)

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 with 10mg/ml Bovine Serum Albumin (BSA, IgG and Protease free) as stabilizer and 0.01% (w/v)Sodium Azide as preservative.Label: Biotinamidocaproate N-Hydroxysuccinimide Ester (BAC). Molar Ratio:

Storage: Store vial at 2-8°C prior to restoration. Centrifuge product if not completely clear afterstanding at room temperature. This product is stable for one month at 2-8°C as anundiluted liquid. For extended storage reconstitute product with 50% glycerol instead of water and thenaliquot contents and freeze at -20°C or below. Dilute only prior to immediate use. Avoid cycles of freezing and thawing. Shelf life: one year from despatch.

Research Area: Enzymes

Restrictions: For Research Use only

Publications

Publications: Wilchek, Bayer: "Labeling glycoconjugates with hydrazide reagents." in: Methods in enzymology, Vol. 138, pp. 429-42, 1987 (PubMed).