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Alkaline Phosphatase antibody

ALP Reactivity: E. coli WB, IP, EIA Host: Rabbit Polyclonal unconjugated
Catalog No. ABIN116596
  • Target See all Alkaline Phosphatase (ALP) Antibodies
    Alkaline Phosphatase (ALP)
    Reactivity
    • 51
    • 23
    • 12
    • 12
    • 11
    • 1
    E. coli
    Host
    • 56
    • 15
    • 4
    • 2
    • 1
    • 1
    Rabbit
    Clonality
    • 54
    • 25
    Polyclonal
    Conjugate
    • 42
    • 12
    • 5
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    This Alkaline Phosphatase antibody is un-conjugated
    Application
    • 50
    • 34
    • 22
    • 19
    • 11
    • 7
    • 7
    • 5
    • 5
    • 4
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    Western Blotting (WB), Immunoprecipitation (IP), Enzyme Immunoassay (EIA)
    Specificity
    This product was prepared from monospecific antiserum by a Delipidation and Defibrination. Assay by immunoelectrophoresis resulted in a single precipitin arc against purified and partially purified Alkaline Phosphatase [E.coli]. No cross reactivity occurs against the mammalian form of Alkaline Phosphatase.
    Purification
    Delipidation and Defibrination
    Immunogen
    Alkaline Phosphatase [E.coli].
    Top Product
    Discover our top product ALP Primary Antibody
  • Application Notes
    Suitable for Immunoblotting (Western or Dot blot), ELISA, Immunoprecipitation and mostImmunological methods requiring high titer and specificity. Note: This product has been assayed against 1.0 μg of Alkaline Phosphatase [E. coli] in astandard sandwich ELISA using Peroxidase conjugated Affinity Purified anti-Rabbit IgG[H&L] (Goat) (Cat#R1364HRP) and (ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonicacid]) as a substrate for 30 minutes at room temperature. A working dilution of 1/20,000 to1/100,000 of the reconstitution concentration is suggested for this product. Recommended Dilutions: ELISA: 1/8,000-1/40,000Western Blot: 1/500-1/2,000
    Other applications not tested.
    Optimal dilutions are dependent on conditions and should be determined by the user.
    Restrictions
    For Research Use only
  • Format
    Liquid
    Reconstitution
    Restore with 2.0 mL of deionized water (or equivalent).
    Concentration
    75.0 mg/mL (by Refractometry)
    Buffer
    0.02 M Potassium Phosphate, 0.12 M Sodium Chloride, pH 7.2 with 0.01 % Sodium Azide as preservative
    Preservative
    Sodium azide
    Precaution of Use
    This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Handling Advice
    Avoid repeated freezing and thawing. Centrifuge product if not completely clear after standing at room temperature.
    Storage
    4 °C/-20 °C
    Storage Comment
    Store vial at 2-8 ° C prior to restoration. Following restoration product can be stored undiluted at 2-8 ° for up to one month or (in aliquots) at -20 °C or below.
  • Target
    Alkaline Phosphatase (ALP)
    Alternative Name
    Alkaline Phosphatase (ALP Products)
    Synonyms
    ALP antibody, ALP1 antibody, CRASH antibody, ECK0378 antibody, JW0374 antibody, psiA antibody, 2410004D18Rik antibody, AU040643 antibody, AW060375 antibody, ILC antibody, CTACK antibody, ESkine antibody, Akp2 antibody, alp antibody, zgc:56672 antibody, DDBDRAFT_0205491 antibody, DDBDRAFT_0231570 antibody, DDB_0205491 antibody, DDB_0231570 antibody, Actn2lp antibody, Alp antibody, asparaginase like 1 antibody, bacterial alkaline phosphatase antibody, C-C motif chemokine ligand 27 antibody, alkaline phosphatase, liver/bone/kidney antibody, alkaline phosphatase antibody, PDZ and LIM domain 3 antibody, ASRGL1 antibody, phoA antibody, Asrgl1 antibody, CCL27 antibody, alpl antibody, alp antibody, Pdlim3 antibody
    Background
    Synonyms: JW0374, b0383, phoA
    Gene ID
    945041
    NCBI Accession
    AP_001034
    UniProt
    P00634
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