Aldolase A, Fructose-Bisphosphate (ALDOA) antibody (Biotin)

Antigen: Aldolase A, Fructose-Bisphosphate (ALDOA)

Synonyms: ALDA, GSD12, MGC10942, MGC17716, MGC17767, Aldo1, Aldo-1, MGC107164, cb79, aldoa, sb:cb79, wu:fa28b10, wu:fb10b11, MGC53030, MGC69273, ALDOA, MGC166216, DKFZp470J0811

Clonality: Polyclonal

Host: Goat

Reactivity: Human, Rabbit

Conjugate: Biotin

Application: Enzyme Immunoassay (EIA), Western Blotting (WB)

Catalog no.: ABIN116609

Additional Information

Characteristics: Synonyms: ALDA, NY-LU-1, Muscle-type aldolase, Fructose-bisphosphate aldolase A, Lung cancerantigen NY-LU-1

Alternative name: Aldolase A / ALDOA

Swiss-Prot: P00883

Immunogen: Aldolase [Rabbit Muscle].

Specificity: This product is an IgG fraction antibody purified from monospecific antiserum by amulti-step process which includes delipidation, salt fractionation and ion exchangechromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Biotin,anti-Goat Serum as well as purified and partially purified Aldolase [Rabbit Muscle]. Cross reactivity against Aldolase from other sources may occur but have not beenspecifically determined.

Application Details

Application Notes: Suitable for Immunoblotting, ELISA, Immunohistochemistry, Immunomicroscopy as well asother antibody based assays using streptavidin or avidin conjugates requiring lot-to-lotconsistency. Recommended Dilutions: This product has been assayed against 1.0 ug of Aldolase in astandard capture ELISA using Peroxidase Conjugated Streptavidin and ABTS(2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes atroom temperature. A working dilution of 1: 4,000 to 1: 16,000 of the reconstitutionconcentration is suggested for this product. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.

Concentration: 10.0 mg/ml (by UV absorbance at 280 nm)

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 with 0.01%sodium azide as preservative and 10 mg/ml Bovine Serum Albumin (BSA, IgG and Proteasefree) as stabilizer.Label: Biotinamidocaproate N-Hydroxysuccinimide Ester (BAC). Molar Ratio: 10-20

Storage: Store vial at 2-8°C prior to restoration. Centrifuge product if not completely clear afterstanding at room temperature. For extended storage reconstitute product with 50%glycerol instead of water and then aliquot contents and freeze at -20°C or below. This product is stable for one month at 2-8°C as an undiluted liquid. Dilute only prior to immediate use. Avoid cycles of freezing and thawing. Shelf life: One year from despatch.

Research Area: Enzymes

Restrictions: For Research Use only

Publications

Publications: Wilchek, Bayer: "Labeling glycoconjugates with hydrazide reagents." in: Methods in enzymology, Vol. 138, pp. 429-42, 1987 (PubMed).