D-Amino-Acid Oxidase (DAO) antibody (Biotin)

Antigen: D-Amino-Acid Oxidase (DAO)

Synonyms: Dao, daao, oxda, damox, dao, DAO, Dao1, DAAO, OXDA, DAMOX, MGC35381, DKFZp469N2232, Dao-1, AI987963, MGC77858, zgc:77858, MGC76928, zgc:76928, SC5F2A.23c, DAO1, MGC113922, wu:fb55b11, zgc:113922, MGC114783

Clonality: Polyclonal

Host: Rabbit

Reactivity: Pig (Porcine)

Conjugate: Biotin

Application: Enzyme Immunoassay (EIA), Western Blotting (WB)

Catalog no.: ABIN116617

Additional Information

Characteristics: Synonyms: DAO, DAMOX, DAAO, OXDA, DAO1

Alternative name: D-amino-acid oxidase

Swiss-Prot: P14920

Immunogen: D-Amino Acid Oxidase [Pig Kidney].

Description: This gene encodes the peroxisomal enzyme D amino acid oxidase. The enzyme is aflavoprotein which uses flavin adenine dinucleotide (FAD) as its prosthetic group. Itssubstrates include a wide variety of D amino acids, but it is inactive on the naturallyoccurring L amino acids. Its biological function is not known, it has been suggested that itis involved in acid base balance in the kidney or it could act as a detoxifying agent whichremoves D-amino acids accumulated during aging, or it may be a fossil enzyme without acurrent function.

Specificity: This product is an IgG fraction antibody purified from monospecific antiserum by amulti-step process which includes delipidation, salt fractionation and ion exchangechromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Biotin,anti-Sheep Serum as well as purified and partially purified D-Amino Acid Oxidase [Pig

Synonyms: Dao, daao, oxda, damox, dao, DAO, Dao1, DAAO, OXDA, DAMOX, MGC35381, DKFZp469N2232, Dao-1, AI987963, MGC77858, zgc:77858, MGC76928, zgc:76928, SC5F2A.23c, DAO1, MGC113922, wu:fb55b11, zgc:113922, MGC114783

Application Details

Application Notes: Suitable for Immunoblotting, ELISA, Immunohistochemistry, Immunomicroscopy as well asother antibody based assays using streptavidin or avidin conjugates requiring lot-to-lotconsistency. Recommended Dilutions: This product has been assayed against 1.0 ug of D-Amino AcidOxidase in a standard capture ELISA using Peroxidase Conjugated Streptavidin and ABTS(2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes atroom temperature. A working dilution of 1: 16,000 to 1: 68,000 of the reconstitutionconcentration is suggested for this product. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.

Concentration: 10.0 mg/ml (by UV absorbance at 280 nm)

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 with 0.01%sodium azide as preservative.Label: Biotinamidocaproate N-Hydroxysuccinimide Ester (BAC). Molar Ratio: 10-20 BAC molecules per Sheep IgG molecule.Reconstitution: Restore with 1.0 ml of

Storage: Store vial at 2-8°C prior to restoration. Centrifuge product if not completely clear afterstanding at room temperature. For extended storage reconstitute product with 50%glycerol instead of water and then aliquot contents and freeze at -20°C or below. This product is stable for one month at 2-8°C as an undiluted liquid. Dilute only prior to immediate use. Avoid cycles of freezing and thawing. Shelf life: One year from despatch.

Research Area: Enzymes

Restrictions: For Research Use only

Publications

Publications: Bayer, Wilchek: "Protein biotinylation." in: Methods in enzymology, Vol. 184, pp. 138-60, 1990 (PubMed).