L-Ascorbate Oxidase antibody (Biotin)

Antigen: L-Ascorbate Oxidase

Synonyms: LOC100281551, LOC100282094, LOC100285297, LOC100285637

Clonality: Polyclonal

Host: Rabbit

Reactivity: Squash (Cucurbita)

Conjugate: Biotin

Application: Enzyme Immunoassay (EIA), Western Blotting (WB)

Catalog no.: ABIN116625

Additional Information

Characteristics: Synonyms: Ascorbase, ASO, AAO

Swiss-Prot: P24792

Immunogen: Ascorbate Oxidase [cucurbita].

Specificity: This product is an IgG fraction antibody purified from monospecific antiserum by amulti-step process which includes delipidation, salt fractionation and ion exchangechromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Biotin,anti-Rabbit Serum as well as purified and partially purified Ascorbate Oxidase [cucurbita]. Cross reactivity against Ascorbate Oxidase from other sources may occur but have notbeen specifically determined.

Application Details

Application Notes: Suitable for Immunoblotting, ELISA, Immunohistochemistry, Immunomicroscopy as well asother antibody based assays using streptavidin or avidin conjugates requiring lot-to-lotconsistency. Recommended Dilutions: This product has been assayed against 1.0 ug of AscorbateOxidase in a standard capture ELISA using Peroxidase Conjugated Streptavidin and ABTS(2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes atroom temperature. A working dilution of 1: 2,000 to 1: 10,000 of the reconstitutionconcentration is suggested for this product. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.

Concentration: 10.0 mg/ml (by UV absorbance at 280 nm)

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 with 0.01%sodium azide as preservative and 10 mg/ml Bovine Serum Albumin (BSA, IgG and Proteasefree) as stabilizer.Label: Biotinamidocaproate N-Hydroxysuccinimide Ester (BAC). Molar Ratio: 10-20

Storage: Store vial at 2-8°C prior to restoration. Centrifuge product if not completely clear afterstanding at room temperature. For extended storage reconstitute product with 50%glycerol instead of water and then aliquot contents and freeze at -20°C or below. This product is stable for one month at 2-8°C as an undiluted liquid. Dilute only prior to immediate use. Avoid cycles of freezing and thawing. Shelf life: One year from despatch.

Research Area: Enzymes

Restrictions: For Research Use only

Publications

Publications: Bayer, Wilchek: "Protein biotinylation." in: Methods in enzymology, Vol. 184, pp. 138-60, 1990 (PubMed).