L-Ascorbate Oxidase antibody (HRP)

Antigen: L-Ascorbate Oxidase

Synonyms: LOC100281551, LOC100282094, LOC100285297, LOC100285637

Clonality: Polyclonal

Host: Rabbit

Reactivity: Squash (Cucurbita)

Conjugate: HRP

Application: Enzyme Immunoassay (EIA), Western Blotting (WB)

Catalog no.: ABIN116626

Additional Information

Characteristics: Synonyms: Ascorbase, ASO, AAO

Swiss-Prot: P24792

Immunogen: Ascorbate Oxidase [cucurbita].

Specificity: This product is an IgG fraction antibody purified from monospecific antiserum by amulti-step process which includes delipidation, salt fractionation and ion exchangechromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc againstanti-Peroxidase, anti-Rabbit Serum and purified and partially purified Ascorbate Oxidase[cucurbita]. Cross reactivity against Ascorbate Oxidase from other sources is unknown.

Application Details

Application Notes: Suitable for Immunoblotting (Western or Dot blot), ELISA, Immunoperoxidase electronmicroscopy and Immunohistochemistryas well as other peroxidase-antibody basedenzymatic assays. Recommended Dilutions: This product has been assayed against 1.0 ug of AlkalinePhosphatase [Calf Intestine] in a standard capture ELISA using ABTS(2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes atroom temperature. A working dilution of 1: 2,000 to 1: 11,000 of the reconstitutionconcentration is suggested for this product. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.

Concentration: 10.0 mg/ml (by UV absorbance at 280 nm)

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 with 0.09%Gentamicin as preservative.Label: Horseradish Peroxidase (HRP).Reconstitution: Restore with 2.0 ml of deionized water (or equivalent).

Storage: Store vial at 2-8°C prior to restoration. Centrifuge product if not completely clear afterstanding at room temperature. For extended storage aliquot contents and freeze at -20°Cor below. This product is stable for one month at 2-8°C as an undiluted liquid. Dilute only prior to immediate use. Avoid cycles of freezing and thawing. Shelf life: One year from despatch. Caution: Do Not Use Sodium Azide ss Preservative.

Research Area: Enzymes

Restrictions: For Research Use only

Publications

Publications: Farr, Nakane: "Immunohistochemistry with enzyme labeled antibodies: a brief review." in: Journal of immunological methods, Vol. 47, Issue 2, pp. 129-44, 1982 (PubMed).