L-Ascorbate Oxidase antibody

Antigen: L-Ascorbate Oxidase

Synonyms: LOC100281551, LOC100282094, LOC100285297, LOC100285637

Clonality: Polyclonal

Host: Rabbit

Reactivity: Squash (Cucurbita)

Application: Enzyme Immunoassay (EIA), Immunoprecipitation (IP), Western Blotting (WB)

Catalog no.: ABIN116627

Additional Information

Characteristics: Synonyms: Ascorbase, ASO, AAO

Swiss-Prot: P24792

Immunogen: Ascorbate Oxidase [cucurbita]

Specificity: This product is an IgG fraction antibody purified from monospecific antiserum by amultistep process which includes delipidation, salt fractionation and ion exchangechromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-RabbitSerum as well as purified and partially purified Ascorbate Oxidase [cucurbita]. Crossreactivity against Ascorbate Oxidase from other sources is unknown.

Application Details

Application Notes: Suitable for ELISA (1/10,000-1/40,000), Western blot (1/2 000-1/10,000),Immunoprecipitation, Conjugation and most immunological methods requiring high titerand specificity. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, with 0.01%(w/v) Sodium Azide as preservative.Reconstitution: Restore with 5.0 ml of deionized water (or equivalent)..

Storage: Store vial at 2-8°C prior to restoration. Centrifuge product if not completely clear afterstanding at room temperature. This product is stable for one month at 2-8°C as anundiluted liquid. For extended storage reconstitute product with 50% glycerol instead of water and thenaliquot contents and freeze at -20°C or below. Dilute only prior to immediate use. Avoid cycles of freezing and thawing. Shelf life: one year from despatch.

Research Area: Enzymes

Restrictions: For Research Use only