Catalase (CAT) antibody (HRP)

Antigen: Catalase (CAT)

Synonyms: MGC138422, MGC138424, Cas1, Cs-1, Cas-1, 2210418N07, CS1, RATCATL, RATCAT01, fb68a12, wu:fb68a12, CAT, CATA, CT21282, DMCATHPO, DROCATHPO, U00145, bs36h11.y1, DmelCG6871, CG6871, MGC128112, CATALASE, CATALASE 2, T12J5.2, Cat, GB11648, catalase, MGC147040, DKFZp469E0232

Clonality: Polyclonal

Host: Rabbit

Reactivity: Cow (Bovine)

Conjugate: HRP

Application: Enzyme Immunoassay (EIA), Western Blotting (WB)

Catalog no.: ABIN116679

Additional Information

Characteristics: Synonyms: CAT, Peroxisome Marker

Alternative name: Catalase

Swiss-Prot: P00432

Immunogen: Catalase from Bovine liver.

Description: Catalase is the classical marker for peroxisomes and is the most abundant protein withinperoxisomes. It is found in almost all aerobically respiring organisms and serves to protectcells from the toxic effects of hydrogen peroxide.

Specificity: This product is an IgG fraction antibody purified from monospecific antiserum by amulti-step process which includes delipidation, salt fractionation and ion exchangechromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against antiPeroxidase, anti-Rabbit Serum as well as purified and partially purified Catalase [Bovineliver]. Cross reactivity against Catalase from other tissues and species may occur but have notbeen specifically determined.

Synonyms: MGC138422, MGC138424, Cas1, Cs-1, Cas-1, 2210418N07, CS1, RATCATL, RATCAT01, fb68a12, wu:fb68a12, CAT, CATA, CT21282, DMCATHPO, DROCATHPO, U00145, bs36h11.y1, DmelCG6871, CG6871, MGC128112, CATALASE, CATALASE 2, T12J5.2, Cat, GB11648, catalase, MGC147040, DKFZp469E0232

Application Details

Application Notes: Suitable for Immunoblotting (Western or Dot blot), ELISA, Immunohistochemistry and mostperoxidase based Immunological methods requiring high titer and specificity. Recommended Dilutions: This product has been assayed against 1.0 ug of Catalase [Bovineliver] in a standard sandwich ELISA using ABTS(2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes atroom temperature. A working dilution of 1: 10,000 to 1: 40,000 of the reconstitutionconcentration is suggested for this product. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.

Concentration: 10.0 mg/ml (by UV absorbance at 280 nm)

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.4 containing10 mg/ml Bovine Serum Albumin (BSA, IgG and Protease free) and 0.01% (w/v) GentamicinSulfate as preservative.Label: Horseradish Peroxidase (HRP).Reconstitution: Restore with 5.0 ml of de

Storage: Store vial at 2-8°C prior to restoration. Centrifuge product if not completely clear afterstanding at room temperature. For extended storage aliquot contents and freeze at -20°Cor below. This product is stable for one month at 2-8°C as an undiluted liquid. Dilute only prior to immediate use. Avoid cycles of freezing and thawing. Shelf life: One year from despatch. Caution: Do Not Use Sodium Azide as Preservative.

Research Area: Cancer, Organelles, Heat Shock Proteins, Cell/Tissue Markers

Restrictions: For Research Use only

Publications

Publications: Farr, Nakane: "Immunohistochemistry with enzyme labeled antibodies: a brief review." in: Journal of immunological methods, Vol. 47, Issue 2, pp. 129-44, 1982 (PubMed).