Glucose Oxidase (GOD) antibody (HRP)

Antigen: Glucose Oxidase (GOD)

Synonyms: LOC406081, GB19418

Clonality: Polyclonal

Host: Rabbit

Conjugate: HRP

Application: Immunohistochemistry (Frozen Sections) (IHC (fro)), Enzyme Immunoassay (EIA), Western Blotting (WB), Immunoelectron Microscopy (EM)

Catalog no.: ABIN116719

Additional Information

Characteristics: Synonyms: Glucose oxyhydrase, Beta-D-glucose: oxygen 1-oxido-reductase, gox

Alternative name: Glucose Oxidase

Swiss-Prot: P13006

Immunogen: Glucose Oxidase [Aspergillus niger]

Description: Glucose Oxidase is widely used for the determination of glucose in body fluids and inremoving residual glucose or oxygen from foods and beverages. Glucose Oxidaseproducing moulds such as Aspergillus and Penicillium species are used for the biologicalproduction of gluconic acid. CATALYTIC ACTIVITY: Beta D glucose + O2 = D glucono 1,5 lactone + H2O2.

Specificity: Assay by immunoelectrophoresis resulted in a single precipitin arc againstanti-Peroxidase, anti-Rabbit Serum as well as purified and partially purified GlucoseOxidase [Aspergillus niger]. Cross reactivity against Glucose Oxidase from other tissuesand species may occur but have not been specifically determined.

Application Details

Application Notes: Immunoblotting (Western or dot blot): 1/1,000 - 1/5,000. ELISA: 1/25,000 - 1/110,000. Immunoperoxidase electron microscopy. Immunohistochemistry as well as other peroxidase-antibody based enzymatic assays: 1/500 - 1/2,500. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.

Concentration: 7.0 mg/ml (by UV absorbance at OD280)

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, containing10 mg/ml Bovine Serum Albumin (BSA, IgG and Protease free) as stabilizer and 0.01%(w/v) Gentamicin Sulfate as preservative.Label: Horseradish Peroxidase (HRP)Reconstitution: Restore wit

Storage: Prior to reconstitution store at 2-8°C. Following reconstitution store the antibody undiluted at 2-8°C for one monthor (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing. Shelf life: one year from despatch.

Research Area: Signaling, Metabolism, Enzymes

Restrictions: For Research Use only

Publications

Publications: Farr, Nakane: "Immunohistochemistry with enzyme labeled antibodies: a brief review." in: Journal of immunological methods, Vol. 47, Issue 2, pp. 129-44, 1982 (PubMed).