Glycerol Kinase antibody

Catalog No. ABIN116737

Product Details anti-Glycerol Kinase Antibody

Target: Glycerol Kinase (GK)

Reactivity: Cellulomonas

Host: Goat

Clonality: Polyclonal

Conjugate: This Glycerol Kinase antibody is un-conjugated

Application: Western Blotting (WB), Enzyme Immunoassay (EIA)

Specificity: Assay by immunoelectrophoresis resulted in a single precipitin arc against purified and partially purified Glycerol Kinase [Cellulomonas species]. Cross reactivity against Glycerol Kinase from other sources is unknown.

Purification: This product was prepared from monospecific antiserum by a delipidation and defibrination.

Immunogen: Glycerol Kinase [Cellulomonas species]

Application Details

Application Notes: Suitable for immunoblotting (western or dot blot), ELISA, immunoprecipitation and mostimmunological methods requiring high titer and specificity. Recommended dilution: This product has been assayed against 1.0 μg of Glycerol Kinase [Celullomonas species] ina standard sandwich ELISA using Peroxidase conjugated Affinity Purified anti-Goat IgG[H&L] (Rabbit) and (ABTS(2,2-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid])as asubstrate for 30 minutes at room temperature. A working dilution of 1: 1,000 to 1: 2,000 of the reconstitution concentration is suggested forthis product.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Format: Liquid

Reconstitution: Restore with 2.0 mL of deionized water or equivalent.

Concentration: 90 mg/mL (by refractometry)

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, containing 0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Dilute only prior to immediate use

Storage: 4 °C

Storage Comment: Store vial at 2-8 °C. DO NOT FREEZE. Freezing alkaline phosphatase conjugates will result in a substantial loss of enzymatic activity.

Target Details for Glycerol Kinase

Target: Glycerol Kinase (GK)

Alternative Name: Glycerol Kinase (GK Products)

Synonyms: GK1 antibody, GKD antibody, D930012N15Rik antibody, GK antibody, CG18374 antibody, Dmel\\CG18374 antibody, dGyk antibody, ASTP antibody, Gyk antibody, gk1 antibody, gk2 antibody, gkd antibody, gyk antibody, Gk antibody, BmGK antibody, DKFZp469P1225 antibody, glycerol kinase antibody, Glycerol kinase 1 antibody, glycerol kinase S homeolog antibody, putative glycerol kinase 3 antibody, ATP:glycerol 3-phosphotransferase; glycerokinase; GK antibody, Glycerol kinase antibody, Fanconi anemia core complex associated protein 100 antibody, GK antibody, Gk antibody, Gk1 antibody, gk.S antibody, LOC705001 antibody, glpK antibody, Spico_1557 antibody, Trebr_0397 antibody, Halhy_5315 antibody, gk antibody, FAAP100 antibody

Background: Glycerol kinase catalyzes the formation of glycerol 3 phosphate from ATP and glycerol. Dihydroxyacetone and L glyceraldehyde can also act as acceptors, UTP and, in the case of the yeast enzyme, ITP and GTP can act as donors. It provides a way for glycerol derived from fats or glycerides to enter the glycolytic pathway. NB - Glycerol kinase obtained from Cellulomonas spp has an observed molecular weight of 128 kDa.Synonyms: ATP:glycerol 3-phosphotransferase, GK, GKD, Glycerokinase

Gene ID: 2710

NCBI Accession: NP_000158

UniProt: P32189