GFP antibody

Catalog No. ABIN116749

Product Details anti-GFP Antibody

Target: GFP (Green Fluorescent Protein (GFP))

Reactivity: Aequorea victoria

Host: Goat

Clonality: Polyclonal

Conjugate: This GFP antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Frozen Sections) (IHC (fro)), Enzyme Immunoassay (EIA)

Specificity: Detects wt GFP, rGFP and eGFP. Assay by Immunoelectrophoresis resulted in a single precipitin arc against anti-Goat serum and purified and partially purified Green Fluorescent Protein (Aequorea victoria) serum. No reaction was observed against Human, Mouse or Rat serum proteins.

Cross-Reactivity (Details): Ads. to Hu, Ms, Rt Serum Proteins

Purification: Immunoaffinity Chromatography using Green Fluorescent Protein (Aequorea victoria) coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities

Immunogen: Green Fluorescent Protein (GFP) fusion protein corresponding to the full length amino acid sequence (246 aa) derived from the jellyfish Aequorea victoria

Application Details

Application Notes: Polyclonal anti-GFP antibody is designed to detect GFP and its variants. This antibody canbe used to detect GFP by ELISA (Sandwich or Capture) for the direct binding of antigen andrecognizes wild type, recombinant and enhanced forms of GFP. Biotin conjugated polyclonal anti-GFP antibody used in a Sandwich ELISA is well suited totitrate GFP in solution using this antibody in combination with a monoclonal anti-GFPantibody (R1461P) using either form of the antibody as the Capture or Detection antibodies. However, use the monoclonal form only for the detection of wild type or recombinant GFP

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL (by UV absorbance at 280 nm)

Buffer: 0.02 M Potassium Phosphate, 0.12 M Sodium Chloride, pH 7.2, 0.01 % (w/v) Sodium Azide, None

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Dilute only prior to immediate use. Avoid cycles of freezing and thawing.

Storage: -20 °C

Storage Comment: Store vial at -20 °C or below prior to opening. Aliquot contents and freeze at -20 °C or below.

References for anti-GFP antibody (ABIN116749)

Ansorg, Bornkessel, Witte, Urbach: "Immunohistochemistry and multiple labeling with antibodies from the same host species to study adult hippocampal neurogenesis." in: Journal of visualized experiments : JoVE, Issue 98, (2015) (PubMed).

Niv, Keiner, Krishna, Witte, Lie, Redecker: "Aberrant neurogenesis after stroke: a retroviral cell labeling study." in: Stroke; a journal of cerebral circulation, Vol. 43, Issue 9, pp. 2468-75, (2012) (PubMed).

Suzuki, Mogami, Ihara, Urano: "Unique secretory dynamics of tissue plasminogen activator and its modulation by plasminogen activator inhibitor-1 in vascular endothelial cells." in: Blood, Vol. 113, Issue 2, pp. 470-8, (2009) (PubMed).

Kofidis, de Bruin, Yamane, Tanaka, Lebl, Swijnenburg, Weissman, Robbins: "Stimulation of paracrine pathways with growth factors enhances embryonic stem cell engraftment and host-specific differentiation in the heart after ischemic myocardial injury." in: Circulation, Vol. 111, Issue 19, pp. 2486-93, (2005) (PubMed).

Hashimoto, Jin, Liu, Chensue, Phan: "Bone marrow-derived progenitor cells in pulmonary fibrosis." in: The Journal of clinical investigation, Vol. 113, Issue 2, pp. 243-52, (2004) (PubMed).

Post, Kajstura, Lei, Sessa, Byrne, Anversa, Hintze, Recchia: "Adeno-associated virus mediated gene delivery into coronary microvessels of chronically instrumented dogs." in: Journal of applied physiology (Bethesda, Md. : 1985), Vol. 95, Issue 4, pp. 1688-94, (2003) (PubMed).

Target Details for GFP

Target: GFP (Green Fluorescent Protein (GFP))

Alternative Name: GFP (GFP Products)

Synonyms: green fluorescent protein antibody, gfp antibody

Target Type: Viral Protein

Background: Green fluorescence protein (GFP) is a 27 kDa protein derived from the jellyfish Aequorea victoria, which emits green light (emission peak at a wavelenth of 509 nm) when excited by blue light (excitation peak at a wavelenth of 395 nm). Green Fluorescent Protein (GFP) has become an invaluable tool in cell biology research, since its intrinsic fluorescence can be visualized in living cells. GFP fluorescence is stable under fixation conditions and suitable for a variety of applications. GFP has been widely used as a reporter for gene expression, enabling researchers to visualize and localize GFP-tagged proteins within living cells without the need for chemical staining. Other applications of GFP include assessment of protein protein interactions through the yeast two hybrid system and measurement of distance between proteins through fluorescence energy transfer (FRET) protocols. GFP technnology has considerably contributed to a greater understanding of cellular physiology. YFP differs from GFP due to a mutation at T203Y, antibodies raised against full-length GFP should also detect YFP and other variants.Synonyms: GFP-Tag, Green fluorescent protein

Gene ID: 6100

UniProt: P42212