Assay by immunoelectrophoresis resulted in a single precipitin arc against purified and partially purified Lactate Dehydrogenase [Rabbit Muscle]. BLAST analysis was used to determine that cross reactivity is suggested for both muscle and heart isoforms (LDH-A and LDH-B) from most mammalian species.
Purification
Prepared from monospecific antiserum by delipidation and defibrination
Immunogen
Full length lactate dehydrogenase protein isolated from Rabbit muscle.
LDHA
Reactivity: Human
WB, ELISA, IHC, IF
Host: Rabbit
Polyclonal
unconjugated
Application Notes
Suitable for use in ELISA (1/4,000-1/20,000) and Western blot (1/500-1/2,000). Expect aband approximately 36 kDa in size corresponding to LDH western blotting in theappropriate cell lysate or extract.
Restrictions
For Research Use only
Format
Liquid
Reconstitution
Restore with 2.0 mL of deionized water (or equivalent)
Concentration
82 mg/mL (by Refractometry)
Buffer
0.02 M Potassium Phosphate, 0.12 M Sodium Chloride, pH 7.2, 0.01 % Sodium Azide, None
Preservative
Sodium azide
Precaution of Use
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice
Avoid repeated freezing and thawing.
Storage
4 °C/-20 °C
Storage Comment
Prior to reconstitution store at 2-8 °C. Following reconstitution store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
Lactate dehydrogenase is also known as L-lactate dehydrogenase A chain, LDH-A, LDH muscle subunit and LDH-M. Two isozymes of LDH occur in mammals, LDH-M and LDH-H which come together to form a homotetramer of 36 kDa subunits. Every LDH molecule consists of four subunits, where each subunit is either H each M (based on their electrophoretic properties.) There are, therefore, five LDH isotypes: LDH-1 (4H) - in the heart, LDH-2 (3H1M) - in the reticuloendothelial system, LDH-3 (2H2M) - in the lungs, LDH-4 (1H3M) - in the kidneys and LDH-5 (4M) - in the liver and striated muscle. Usually LDH-2 is the predominant form in the serum. An LDH-1 level higher than the LDH-2 level (a "flipped pattern") suggests myocardial infarction (damage to heart tissues releases heart LDH, which is rich in LDH-1, into the bloodstream). In general, LDH is often used as a marker of tissue breakdown. LDH shows a cytoplasmic localization.Synonyms: Cell proliferation-inducing gene 19 protein, L-lactate dehydrogenase A chain, LDH muscle subunit, LDH-A, LDH-M, NY-REN-59, PIG19, Renal carcinoma antigen NY-REN-59