MDH1
(Malate Dehydrogenase 1, NAD (Soluble) (MDH1))
Reactivity
Pig
Host
Sheep
Clonality
Polyclonal
Conjugate
This MDH1 antibody is un-conjugated
Application
Western Blotting (WB), Enzyme Immunoassay (EIA)
Specificity
Assay by immunoelectrophoresis resulted in a single precipitin arc against purified and partially purified Malate Dehydrogenase [Pig Heart]. Cross reactivity against Malate Dehydrogenase from other tissues and species may occur but have not been specifically determined.
Purification
Prepared from monospecific antiserum by a delipidation and defibrination.
Suitable for Immunoblotting (Western or Dot blot), ELISA, Immunoprecipitation and mostimmunological methods requiring high titer and specificity. Recommended Dilutions: This product has been assayed against 1.0 μg of MalateDehydrogenase [Pig Heart] in a standard sandwich ELISA using Peroxidase conjugatedAffinity Purified anti-Sheep IgG [H&L] (Goat) and ABTS(2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes atroom temperature. A working dilution of 1: 3,000 to 1: 12,000 of the reconstitutionconcentration is suggested for this product. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions
For Research Use only
Format
Liquid
Reconstitution
Restore with 2.0 mL of deionized water (or equivalent).
Concentration
80.0 mg/mL (by Refractometry)
Buffer
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 with 0.01 % Sodium Azide as preservative.
Preservative
Sodium azide
Precaution of Use
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice
Avoid repeated freezing and thawing. Dilute only prior to immediate use
Storage
4 °C/-20 °C
Storage Comment
Store vial at 2-8 °C prior to restoration. For extended storage add glycerol to 50% and then aliquot contents and freeze at -20 °C or below. Centrifuge product if not completely clear after standing at room temperature. This antibody is stable for one month at 2-8 °C as an undiluted liquid.
Target
MDH1
(Malate Dehydrogenase 1, NAD (Soluble) (MDH1))
Malate dehydrogenase catalyzes the interconversion of L-malate and oxaloacetate using nicotinamide adenine dinucleotide (NAD) as a coenzyme. Malate dehydrogenase is found in all eukaryotic cells as two isozymes: mitochondrial (m-MDH) and cytoplasmic (soluble, s-MDH). Prokaryotes contain only a single form. The two isozymes, both consisting of two very similar subunits of about 35kD and having similar enzymatic activity appear as different proteins. There is structural similarity of mitochondrial malate dehydrogenase to L-3-hydroxyacyl CoA dehydrogenase and the cytoplasmic malate dehydrogenase to lactate dehydrogenase.Synonyms: MDH1, Malate dehydrogenase, cytoplasmic