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MDH1 antibody

MDH1 Reactivity: Pig WB, EIA Host: Sheep Polyclonal unconjugated
Catalog No. ABIN116777
  • Target See all MDH1 Antibodies
    MDH1 (Malate Dehydrogenase 1, NAD (Soluble) (MDH1))
    Reactivity
    • 55
    • 27
    • 26
    • 8
    • 3
    • 3
    • 3
    • 3
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    Pig
    Host
    • 45
    • 11
    • 4
    • 1
    Sheep
    Clonality
    • 50
    • 10
    Polyclonal
    Conjugate
    • 39
    • 5
    • 3
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    This MDH1 antibody is un-conjugated
    Application
    • 58
    • 25
    • 17
    • 13
    • 13
    • 12
    • 12
    • 9
    • 8
    • 7
    • 6
    • 4
    • 4
    • 3
    • 2
    • 2
    • 2
    • 1
    Western Blotting (WB), Enzyme Immunoassay (EIA)
    Specificity
    Assay by immunoelectrophoresis resulted in a single precipitin arc against purified and partially purified Malate Dehydrogenase [Pig Heart]. Cross reactivity against Malate Dehydrogenase from other tissues and species may occur but have not been specifically determined.
    Purification
    Prepared from monospecific antiserum by a delipidation and defibrination.
    Immunogen
    Malate Dehydrogenase [Pig Heart].
    Top Product
    Discover our top product MDH1 Primary Antibody
  • Application Notes
    Suitable for Immunoblotting (Western or Dot blot), ELISA, Immunoprecipitation and mostimmunological methods requiring high titer and specificity. Recommended Dilutions: This product has been assayed against 1.0 μg of MalateDehydrogenase [Pig Heart] in a standard sandwich ELISA using Peroxidase conjugatedAffinity Purified anti-Sheep IgG [H&L] (Goat) and ABTS(2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes atroom temperature. A working dilution of 1: 3,000 to 1: 12,000 of the reconstitutionconcentration is suggested for this product.
    Other applications not tested.
    Optimal dilutions are dependent on conditions and should be determined by the user.
    Restrictions
    For Research Use only
  • Format
    Liquid
    Reconstitution
    Restore with 2.0 mL of deionized water (or equivalent).
    Concentration
    80.0 mg/mL (by Refractometry)
    Buffer
    0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 with 0.01 % Sodium Azide as preservative.
    Preservative
    Sodium azide
    Precaution of Use
    This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Handling Advice
    Avoid repeated freezing and thawing. Dilute only prior to immediate use
    Storage
    4 °C/-20 °C
    Storage Comment
    Store vial at 2-8 °C prior to restoration. For extended storage add glycerol to 50% and then aliquot contents and freeze at -20 °C or below. Centrifuge product if not completely clear after standing at room temperature. This antibody is stable for one month at 2-8 °C as an undiluted liquid.
  • Target
    MDH1 (Malate Dehydrogenase 1, NAD (Soluble) (MDH1))
    Alternative Name
    MDHA (MDH1 Products)
    Synonyms
    MDH-s antibody, MDHA antibody, MGC:1375 antibody, MOR2 antibody, ODS1 antibody, B230377B03Rik antibody, D17921 antibody, Mor-2 antibody, Mor2 antibody, MDL1 antibody, Mdhl antibody, MDH antibody, mdh-s antibody, mdha antibody, mor2 antibody, MDH-1 antibody, Mdh antibody, Mdh-1 antibody, Mdh2 antibody, MdhD antibody, cMdh antibody, sMdh antibody, malate dehydrogenase 1 antibody, malic enzyme 2 antibody, malate dehydrogenase 1, NAD (soluble) antibody, malate dehydrogenase 1 S homeolog antibody, malate dehydrogenase, cytoplasmic antibody, Malate dehydrogenase 1 antibody, MDH1 antibody, ME2 antibody, Mdh1 antibody, mdh1.S antibody, LOC100280767 antibody
    Background
    Malate dehydrogenase catalyzes the interconversion of L-malate and oxaloacetate using nicotinamide adenine dinucleotide (NAD) as a coenzyme. Malate dehydrogenase is found in all eukaryotic cells as two isozymes: mitochondrial (m-MDH) and cytoplasmic (soluble, s-MDH). Prokaryotes contain only a single form. The two isozymes, both consisting of two very similar subunits of about 35kD and having similar enzymatic activity appear as different proteins. There is structural similarity of mitochondrial malate dehydrogenase to L-3-hydroxyacyl CoA dehydrogenase and the cytoplasmic malate dehydrogenase to lactate dehydrogenase.Synonyms: MDH1, Malate dehydrogenase, cytoplasmic
    Gene ID
    396894
    NCBI Accession
    NP_999039
    UniProt
    P11708
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