Ovalbumin antibody

Catalog No. ABIN116785

Product Details anti-Ovalbumin Antibody

Target: Ovalbumin (OVA)

Reactivity: Chicken

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Ovalbumin antibody is un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP), Enzyme Immunoassay (EIA)

Specificity: Assay by Immunoelectrophoresis resulted in a single precipitin arc against purified and partially purified Ovalbumin [Hen Egg White]. Cross reactivity against Ovalbumin from other tissues and species may occur but have not been specifically determined.

Purification: Delipidation and Defibrination

Immunogen: Ovalbumin from Hen Egg White.

Application Details

Application Notes: Suitable for Immunoblotting (Western: 1/500-1/5000 or Dot blot), ELISA (1/5000-1/20000),Immunoprecipitation (1/100) and most Immunological methods requiring high titer andspecificity. Recommended Dilutions: This product has been assayed against 1.0 μg of Ovalbumin [Hen Egg White] in a standardsandwich ELISA using Peroxidase conjugated Affinity Purified anti-Rabbit IgG [H&L] (Goat)(Cat#R1364HRP) and ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as asubstrate for 30 minutes at room temperature. A working dilution of 1/90,000 to 1/450,000of the reconstitution concentration is suggested for this product.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Format: Liquid

Reconstitution: Restore with 2.0 mL of deionized water (or equivalent).

Concentration: 80.0 mg/mL (by Refractometry)

Buffer: 0.02 M Potassium Phosphate, 0.12 M Sodium Chloride, pH 7.2, 0.01 % (w/v) Sodium Azide, None

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Prior to reconstitution store at 2-8 °C. Following reconstitution store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for Ovalbumin

Target: Ovalbumin (OVA)

Alternative Name: Ovalbumin (OVA Products)

Synonyms: 1600019A21Rik antibody, R86 antibody, SPI-CI antibody, Spi10 antibody, ovalbumin antibody, NK13 antibody, Spi12 antibody, BC052216 antibody, NK21 antibody, NK21L1 antibody, Spi13 antibody, NK26 antibody, Spi14 antibody, AW540195 antibody, AT2 antibody, Spi9 antibody, EID antibody, SPI3B antibody, Gm11396 antibody, OTTMUSG00000000720 antibody, SERPINB14 antibody, C76171 antibody, C76174 antibody, SPI3C antibody, SPIC antibody, serine (or cysteine) peptidase inhibitor, clade B, member 9b antibody, serine (or cysteine) peptidase inhibitor, clade B, member 6b antibody, serine (or cysteine) peptidase inhibitor, clade B, member 9f antibody, serine (or cysteine) peptidase inhibitor, clade B, member 9e antibody, serine (or cysteine) peptidase inhibitor, clade B, member 9d antibody, serine (or cysteine) peptidase inhibitor, clade B, member 1, pseudogene antibody, serine (or cysteine) peptidase inhibitor, clade B, member 6e antibody, ovalbumin (SERPINB14) antibody, serine (or cysteine) peptidase inhibitor, clade B, member 6c antibody, Serpinb9b antibody, Serpinb6b antibody, Serpinb9f antibody, Serpinb9e antibody, Serpinb9d antibody, Serpinb1-ps1 antibody, Serpinb6e antibody, OVAL antibody, Serpinb6c antibody

Background: Chicken Ovalbumin is the major protein in the "white" of the egg (and a favorite antigen in immunological research). Egg white contains a variety of proteins including ovalbumin, conalbumin, ovomucoid and lysozyme. It belongs to the serpin family and the Ov serpin subfamily. Ovalbumin can cause an allergic reaction in humans. Ovalbumin has been implicated in the development of the egg shell. Immunohistochemistry revealed that ovalbumin is found only in the mammillary bodies of decalcified shell, and is not distributed throughout the shell matrix. These results indicate that ovalbumin is present during the initial phase of shell formation and becomes incorporated into the protein matrix of the mammillary bodies. However, it is not yet clear whether ovalbumin at this site plays a specific role in shell mineralisation. Anti Ovalbumin Antibody recognizes ovalbumin that is the main protein found in egg white. Ovalbumin makes up 60-65 % of the total protein. Ovalbumin displays sequence and three-dimensional homology to the serpin superfamily, but unlike most serpins it is not a serine protease inhibitor. The function of ovalbumin is unknown, although it is presumed to be a storage protein.Synonyms: Allergen Gal d II, Egg albumin, Plakalbumin, SERPINB14

Gene ID: 396058

NCBI Accession: NP_990483

UniProt: P01012