Pepsin antibody (Biotin)
| Antigen |
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| Clonality |
Polyclonal |
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Host
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Reactivity
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Conjugate
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Application
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 Alternatives Enzyme Immunoassay (EIA), Western Blotting (WB)
Western Blotting (WB) (27), ELISA (22), Immunoprecipitation (IP) (14), Enzyme Immunoassay (EIA) (7), Immunofluorescence (IF) (7), Immunohistochemistry (IHC) (7), Dot Blot (Dot) (3), Immunodiffusion (ID) (3), Radioimmunoassay (RIA) (3), Immunoelectrophoresis (IEP) (2), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (2), Immunohistochemistry (Frozen Sections) (IHC (fro)) (1)
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| Catalog no. |
ABIN116803 |
| Quantity |
10 mg (10.0 mg/ml (by UV absorbance at 280...) |
| Price |
310.00 $ Plus shipping costs $35.00
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| Shipping to |
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| Availability |
Ships within 7 to 10 Business Days |
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Characteristics
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Synonyms: Pepsin A, PGA, pepsinogen 3 group I, pepsinogen 4 group I, pepsinogen 5 group I,Pepsinogen A, PGA3, PGA4, PGA5
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Gene ID
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396892
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Swiss-Prot
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P00791
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Immunogen
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Pepsin [Porcine Stomach].
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Specificity
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This product is an IgG fraction antibody purified from monospecific antiserum by amulti-step process which includes delipidation, salt fractionation and ion exchangechromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Biotin,anti-Goat Serum as well as purified and partially purified Pepsin [Porcine Stomach]. Cross reactivity against Pepsin from other sources may occur but have not beenspecifically determined.
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Application Notes
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Suitable for Immunoblotting, ELISA, Immunohistochemistry, Immunomicroscopy as well asother antibody based assays using streptavidin or avidin conjugates requiring lot-to-lotconsistency. Recommended Dilutions: This product has been assayed against 1.0 ug of Pepsin in astandard capture ELISA using Peroxidase Conjugated Streptavidin and ABTS(2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes atroom temperature. A working dilution of 1: 3,000 to 1: 15,000 of the reconstitutionconcentration is suggested for this product. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
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Concentration
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10.0 mg/ml (by UV absorbance at 280 nm)
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Buffer
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0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 with 0.01%(w/v) Sodium Azide as preservative and 10 mg/ml Bovine Serum Albumin (BSA, IgG andProtease free) as stabilizer.Label: Biotinamidocaproate N-Hydroxysuccinimide Ester (BAC). Molar Ratio:
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Storage
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Store vial at 2-8°C prior to restoration. For extended storage add glycerol to 50% and thenaliquot contents and freeze at -20°C or below. Centrifuge product if not completely clearafter standing at room temperature. This antibody is stable for one month at 2-8°C as an undiluted liquid. Dilute only prior to immediate use. Avoid repeated freezing and thawing. Shelf life: One year from despatch.
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Research Area
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Signaling, Metabolism, Amino Acids, Proteases
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Restrictions
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For Research Use only
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Publications
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Wilchek, Bayer: "Labeling glycoconjugates with hydrazide reagents." in: Methods in enzymology, Vol. 138, pp. 429-42, 1987 (PubMed).
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Alternatives for antigen "Pepsin", type "Antibodies"
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Hosts
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Goat (22), Rabbit (7)
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Reactivities
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Pig (Porcine) (18), Human (1)
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Applications
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Western Blotting (WB) (27), ELISA (22), Immunoprecipitation (IP) (14), Enzyme Immunoassay (EIA) (7), Immunofluorescence (IF) (7), Immunohistochemistry (IHC) (7), Dot Blot (Dot) (3), Immunodiffusion (ID) (3), Radioimmunoassay (RIA) (3), Immunoelectrophoresis (IEP) (2), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (2), Immunohistochemistry (Frozen Sections) (IHC (fro)) (1)
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Conjugates
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Biotin (7), HRP (5)
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