PIPOX antibody
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- Target See all PIPOX Antibodies
- PIPOX (Pipecolic Acid Oxidase (PIPOX))
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Reactivity
- Bacteria
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Host
- Goat
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Clonality
- Polyclonal
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Conjugate
- This PIPOX antibody is un-conjugated
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Application
- Western Blotting (WB), Immunoprecipitation (IP), Enzyme Immunoassay (EIA)
- Specificity
- Assay by immunoelectrophoresis resulted in a single precipitin arc against purified and partially purified Sarcosine Oxidase [Microbial]. Cross reactivity against Sarcosine Oxidase from other sources is unknown.
- Purification
- Prepared from monospecific antiserum by a delipidation and defibrination.
- Immunogen
- Sarcosine Oxidase [Microbial].
- Top Product
- Discover our top product PIPOX Primary Antibody
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- Application Notes
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Suitable for Immunoblotting (Western or Dot blot), ELISA, Immunoprecipitation and mostimmunological methods requiring high titer and specificity. Recommended Dilutions: This product has been assayed against 1.0 μg of SarcosineOxidase [Microbial] in a standard sandwich ELISA using Peroxidase conjugated AffinityPurified anti-Goat IgG [H&L] (Rabbit) and ABTS(2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes atroom temperature. A working dilution of 1: 4,000 to 1: 16,000 of the reconstitutionconcentration is suggested for this product.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. - Restrictions
- For Research Use only
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- Format
- Liquid
- Reconstitution
- Restore with 2.0 mL of deionized water (or equivalent).
- Concentration
- 90.0 mg/mL (by Refractometry)
- Buffer
- 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 with 0.01 % sodium azide as preservative.
- Preservative
- Sodium azide
- Precaution of Use
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Handling Advice
- Avoid repeated freezing and thawing. Dilute only prior to immediate use
- Storage
- 4 °C/-20 °C
- Storage Comment
- Store vial at 2-8 °C prior to restoration. For extended storage add glycerol to 50% and then aliquot contents and freeze at -20 °C or below. Centrifuge product if not completely clear after standing at room temperature. This antibody is stable for one month at 2-8 °C as an undiluted liquid.
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- Target
- PIPOX (Pipecolic Acid Oxidase (PIPOX))
- Alternative Name
- Sarcosine Oxidase (PIPOX Products)
- Synonyms
- LPIPOX antibody, Pso antibody, SOX antibody, pipecolic acid and sarcosine oxidase antibody, pipecolic acid oxidase antibody, pipecolic acid oxidase L homeolog antibody, PIPOX antibody, Pipox antibody, pipox antibody, pipox.L antibody
- Background
- Synonyms: L-pipecolate oxidase, L-pipecolic acid oxidase, LPIPOX, PIPOX, PSO, Peroxisomal sarcosine oxidase
- Gene ID
- 51268
- NCBI Accession
- NP_057602
- UniProt
- Q9P0Z9
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