Urease (URE) antibody
| Antigen |
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| Synonyms |
F12B7.10, F12B7_10, URE, UREASE |
| Clonality |
Polyclonal |
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Host
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Application
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 Alternatives Enzyme Immunoassay (EIA), Immunoprecipitation (IP), Western Blotting (WB)
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| Catalog no. |
ABIN116845 |
| Quantity |
2 ml (90.0 mg/ml (by Refractometry)) (Variants) |
| Price |
350.00 $ Plus shipping costs $35.00
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| Shipping to |
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| Availability |
Ships within 7 to 10 Business Days |
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Characteristics
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Synonyms: Urea amidohydrolase
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Alternative name
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Urease
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Swiss-Prot
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P07374
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Immunogen
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Urease [Jack Bean].
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Format
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Serum
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Specificity
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Assay by immunoelectrophoresis resulted in a single precipitin arc against purified andpartially purified Urease [Jack Bean]. Cross reactivity against Urease from other tissues and species may occur but have notbeen specifically determined.
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Application Notes
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Suitable for Immunoblotting (Western or Dot blot), ELISA, Immunoprecipitation and mostimmunological methods requiring high titer and specificity. Recommended Diutions: This product has been assayed against 1.0 ug of Urease [JackBean] in a standard sandwich ELISA using Peroxidase conjugated Affinity Purifiedanti-Rabbit IgG [H&L] (Goat) and (ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonicacid]) as a substrate for 30 minutes at room temperature. A working dilution of 1: 1,000 to1: 3,000 of the reconstitution concentration is suggested for this product. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
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Concentration
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90.0 mg/ml (by Refractometry)
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Buffer
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0.01 M Sodium Phosphate, 0.14 M Sodium Chloride, pH 7.4 with 0.01%sodium azide as preservative.Reconstitution: Restore with 2.0 ml of deionized water (or equivalent).
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Storage
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Store vial at 2-8°C prior to restoration. For extended storage add glycerol to 50% and thenaliquot contents and freeze at -20°C or below. Centrifuge product if not completely clearafter standing at room temperature. This antibody is stable for one month at 2-8°C as an undiluted liquid. Dilute only prior to immediate use. Avoid repeated freezing and thawing. Shelf life: One year from despatch.
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Research Area
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Enzymes, Metabolism
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Restrictions
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For Research Use only
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Alternatives for antigen "Urease (URE)", type "Antibodies"
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Hosts
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Rabbit (29)
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Reactivities
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Jack Bean (9), Jackbean (Canavalia ensiformis) (3), Plant (2)
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Applications
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Western Blotting (WB) (25), ELISA (24), Immunoprecipitation (IP) (12), Immunofluorescence (IF) (9), Immunohistochemistry (IHC) (7), Enzyme Immunoassay (EIA) (5), Dot Blot (Dot) (1), Flow Cytometry (FACS) (1), Immunodiffusion (ID) (1), Radioimmunoassay (RIA) (1)
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Conjugates
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HRP (8), Biotin (7)
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