Urease antibody

Catalog No. ABIN116845

This Rabbit Polyclonal antibody specifically detects Urease in WB, IP and EIA. It exhibits reactivity toward Jack Bean.

Quick Overview for Urease antibody (ABIN116845)

Target: Urease (URE)

Reactivity: Jack Bean

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Urease antibody is un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP), Enzyme Immunoassay (EIA)

Product Details anti-Urease Antibody

Purification: Prepared from monospecific antiserum by a Delipidation and Defibrination

Immunogen: Urease [Jack Bean].

Application Details

Application Notes: Suitable for Immunoblotting (Western or Dot blot), ELISA, Immunoprecipitation and mostimmunological methods requiring high titer and specificity. Recommended Diutions: This product has been assayed against 1.0 μg of Urease [JackBean] in a standard sandwich ELISA using Peroxidase conjugated Affinity Purifiedanti-Rabbit IgG [H&L] (Goat) and (ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonicacid]) as a substrate for 30 minutes at room temperature. A working dilution of 1: 1,000 to1: 3,000 of the reconstitution concentration is suggested for this product.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Format: Liquid

Reconstitution: Restore with 2.0 mL of deionized water (or equivalent).

Concentration: 90.0 mg/mL (by Refractometry)

Buffer: 0.01 M Sodium Phosphate, 0.11 M Sodium Chloride, pH 7.4, 0.01 % Sodium Azide, None

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Prior to reconstitution store at 2-8 °C. Following reconstitution store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for Urease

Target: Urease (URE)

Alternative Name: Urease

Background: Urease is a protein that is commonly secreted from the bacterium H. Pylori and is integral to the immune response within the gastric system. Urease carries out a key enzymatic reaction in converting urea into free ammonia and carbonic acid. Urease is commonly derived from the Jack Bean plant in scientific research as the amino acid sequences are highly conserved between species and it is an abundant source of the protein. Anti-Urease Antibody is ideal for investigators in Immunology, Enzymology, and Cell Biology research.Synonyms: Urea amidohydrolase

Gene ID: 3823

UniProt: P07374