Dihydrolipoamide Dehydrogenase (DLD) antibody (Biotin)

Antigen: Dihydrolipoamide Dehydrogenase (DLD)

Synonyms: E3, LAD, DLDH, GCSL, PHE3, AI315664, AI746344, wu:fb24b05, DLD, DKFZp459I0911, ABI-2, ABI2B, AIP-1, AblBP3, SSH3BP2, argBPIA, argBPIB

Clonality: Polyclonal

Host: Rabbit

Reactivity: Pig (Porcine)

Conjugate: Biotin

Application: Enzyme Immunoassay (EIA), Western Blotting (WB)

Catalog no.: ABIN116858

Additional Information

Characteristics: Synonyms: DLD, GCSL, LAD, PHE3, EC 1.8.1.4, Dihydrolipoamide dehydrogenase, Glycine cleavagesystem L protein

Alternative name: Dihydrolipoyl dehydrogenase

Swiss-Prot: P09622

Immunogen: Lipoamide Dehydrogenase [Porcine Heart]

Description: Lipoamide dehydrogenase or dihydrolipoamide dehydrogenase (DLD) is a component ofthe glycine cleavage system as well as of the alpha ketoacid dehydrogenase complexes. Itis a homodimer. It belongs to the class I pyridine nucleotide disulfide oxidoreductasefamily. Defects in DLD are a cause of congenital infantile lactic acidosis. Moreover, defects in DLD are the cause of DLD deficiency, which results in extensivemetabolic disturbances, including lactic acidemia, Krebs cycle dysfunction, and impairedbranched amino acid degradation. DLD deficiency also causes neurological degenerationdue to the sensitivity of the central nervous system to defects in oxidative metabolism.

Specificity: This product is an IgG fraction antibody purified from monospecific antiserum by amultistep process which includes delipidation, salt fractionation and ion exchangechromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Biotin,anti-Rabbit Serum as well as purified and partially purified Lipoamide Dehydrogenase[Porcine Heart]. Cross reactivity against Lipoamide Dehydrogenase from other sources may occur but havenot been specifically determined. Species: Pig. Others not tested.

Application Details

Application Notes: Suitable for Immunoblotting (Western or Dot blot), ELISA, Immunoperoxidase ElectronMicroscopy and Immunohistochemistry as well as other peroxidase-antibody basedenzymatic assays. Note: This product has been assayed against 1.0 µg of Lipoamide Dehydrogenase in astandard Capture ELISA using Peroxidase Conjugated Streptavidin and ABTS(2,2’-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at RT. A working dilution of 1/4,000-1/20,000 of the reconstitution concentration is suggested forthis product. Recommended Dilutions: ELISA: 1/5,000-1/20,000Western Blot: 1/500-1/5,000

Concentration: 10.0 mg/ml (by UV absorbance at 280 nm)

Buffer: 0.02M Potassium Phosphate, 0.15M Sodium Chloride, pH 7.2, with 10mg/ml BSA (IgG and Protease free) as stabilizer and 0.01% (w/v) Sodium Azide aspreservative.Label: Biotinamidocaproate N-Hydroxysuccinimide Ester (BAC). Molar Ratio: 10-20 BAC molecules per

Storage: Store vial at 2-8°C prior to restoration. For extended storage add glycerol to 50% and thenaliquot contents and freeze at -20°C or below. Centrifuge product if not completely clearafter standing at room temperature. This antibody is stable for one month at 2-8°C as an undiluted liquid. Dilute only prior to immediate use. Avoid repeated freezing and thawing. Shelf life: One year from despatch.

Research Area: Enzymes, Metabolism

Restrictions: For Research Use only

Publications

Publications: Wilchek, Bayer: "Labeling glycoconjugates with hydrazide reagents." in: Methods in enzymology, Vol. 138, pp. 429-42, 1987 (PubMed).