IL-1 beta antibody

Catalog No. ABIN116862

Product Details anti-IL-1 beta Antibody

Target: IL-1 beta (IL1B) (Interleukin 1, beta (IL1B))

Reactivity: Human, Monkey

Host: Rabbit

Clonality: Polyclonal

Conjugate: This IL-1 beta antibody is un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA), Functional Studies (Func), Radioimmunoassay (RIA), Immunohistochemistry (Frozen Sections) (IHC (fro))

Specificity: This antibody is primarily directed against mature, 17,000 MW human IL1 beta and is useful in determining its presence in various assays. In general, this antibody also detects primate IL-1b in the same formats using similar dilutions. The antiserum does not recognize human IL-1a. In ELISA formats and other immunoreactive assays, this antibody will recognize 10 % of the non-denatured (native) precursor 31,000 MW IL-1b containing samples but will primarily detect all of the 17,000 MW mature molecule. However, in immunoblot analysis of natural cell products or human body fluids, the usual procedure of heating the sample in SDS with or without reducing agents will facilitate denaturing of the 31,000 MW IL1b precursor molecule. Denatured 31,000 precursor IL-1b will be recognized by this antibody but often migrates as a 35,000 MW band. This is due to the unfolding of the denatured precursor IL-1b exposing epitopes not exposed in the natural state. In immunoblots, depending on the number of cells, a 1:100 to 1:200 dilution of this antiserum detected the 17,000 MW band in supernatants as well as a 35,000 MW band representing the 31,000 MW IL-1b precursor in lysates. This antibody is also useful for neutralization of human and primate IL-1b activity in bioassays. It does not neutralize the biological activity IL-1a. It does not neutralize the biological activity of murine, rat or rabbit IL-1b . It will neutralize primate IL-1b. For neutralization, it is recommended to incubate the sample with a 1:100 dilution of the antiserum for at least 4 hours before being tested. A control of similarly diluted normal rabbit IgG is recommended. This antibody can be used for FACS analysis. Caution should be exhibited as the F(c) domain of the rabbit IgG molecule may interact with cells non-specifically.

Purification: DEAE fractionation.

Immunogen: The whole rabbit serum used to produce this IgG fraction antibody was prepared by repeated immunizations with recombinant 153 aa human IL1 beta produced in E.coli. Remarks: The MW of the recombinant IL-1b was 17,000 with the N-terminal amino acid at position alanine 117. This is the cleavage site generated by the IL1 beta converting enzyme (ICE, capase-1).

Application Details

Application Notes: This IgG fraction of anti-Human IL1 beta antibody has been tested for use inNeutralizations, ELISA, Radioimmunoassays, Flow cytometry, Immunohistochemistry,Immunoblotting and Immunoprecipitation. It recognizes the 17,000 MW mature IL1 beta. Recommended Dilutions: Neutralization: 1/100Radioimmunoassay: 1/5,000-1/10,000Western blot: 1/1,000, in Immunoblots, typically, IL1 beta is detected from supernatants orlysates of 2 x 10^6 endotoxin-stimulated human peripheral blood mononuclear cells(PBMC). PBMC are stimulated for 24 hours with 1 % (v/v) human serum plus 10 ng/mL E. coliLPS. Immunoprecipitation: 1/400-1/800, Pre- clearing the preparation with a non-specific rabbitIgG to reduce background. Immunohistochemistry: 1/100-1/200, either Paraffin fixation or cryofixation can be used for

Restrictions: For Research Use only

Handling

Concentration: 2.0 mg/mL (by UV absorbance at 280 nm)

Buffer: 0.02 M Potassium Phosphate, 0.12 M Sodium Chloride, pH 7.2 without preservatives or stabilizers.

Preservative: Without preservative

Handling Advice: Avoid repeated freezing and thawing. This product is photosensitive and should be protected from light.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for IL-1 beta

Target: IL-1 beta (IL1B) (Interleukin 1, beta (IL1B))

Alternative Name: Interleukin-1 beta / IL-1B (IL1B Products)

Synonyms: IL-1 antibody, IL1-BETA antibody, IL1F2 antibody, IL-1BETA antibody, IL1beta antibody, il1-b antibody, zgc:111873 antibody, IL-1B antibody, IL-1beta antibody, Il-1b antibody, IL1B antibody, IL-1 beta antibody, IL-1b antibody, interleukin 1 beta antibody, interleukin 1, beta antibody, IL1B antibody, il1b antibody, Il1b antibody

Background: Interleukin 1 (IL1), originally known as lymphocyte activating factor (LAF), activates T cells and lymphocytes, which then proliferate and secrete interleukin 2. IL1 is primarily released from stimulated macrophages and monocytes, but also is released from several other cell types, and is thought to play a key role in inflammatory and immune responses. The two closely related agents, interleukin1 alpha (IL1 alpha) and interleukin1 beta (IL1 beta) bind to the same cell surface receptor, elicit nearly identical biological responses and share 25 % homology in their amino acid sequence.Synonyms: Catabolin, IL-1 beta, IL1 beta, IL1B, IL1F2

Gene ID: 3553

NCBI Accession: NP_000567

UniProt: P01584

Pathways: NF-kappaB Signaling, Interferon-gamma Pathway, TLR Signaling, Negative Regulation of Hormone Secretion, Cellular Response to Molecule of Bacterial Origin, Carbohydrate Homeostasis, Glycosaminoglycan Metabolic Process, Myometrial Relaxation and Contraction, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Autophagy, Cancer Immune Checkpoints, Inflammasome