GM-CSF antibody

Antigen: GM-CSF

Synonyms: GMCSF, GM-CSF

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Application: Immunohistochemistry (Frozen Sections) (IHC (fro)), Enzyme Immunoassay (EIA), Functional Studies (Func), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)

Catalog no.: ABIN116866

Additional Information

Characteristics: Synonyms: CSF2, GMCSF, Sargramostim, Molgramostin, Granulocyte-macrophage colony-stimulatingfactor, Colony stimulating factor 2

Swiss-Prot: P04141

Immunogen: This whole rabbit serum dy was prepared by repeated immunizations with recombinanthuman GM-CSF produced in Chinese Hamster cells.

Format: Serum

Description: Granulocyte Macrophage Colony Stimulating Factor (also known as GM-CSF,Colony-stimulating factor, CSF, sargramostim and molgramostin) is produced in responseto a number of inflammatory mediators by mesenchymal cells present in the hemopoieticenvironment and at peripheral sites of inflammation. Granulocyte Macrophage-CSF is ableto stimulate the production of neutrophilic granulocytes, macrophages, and mixedgranulocyte-macrophage colonies from bone marrow cells and can stimulate the formationof eosinophil colonies from fetal liver progenitor cells. GM-CSF can also stimulate somefunctional activities in mature granulocytes and macrophages. GM-CSF receptors showsignificant homologies with other receptors for hematopoietic growth factors, includingIL2-beta, IL-3, IL-6, IL-7, EPO and the Prolactin receptors.

Specificity: This antiserum has been heated to 56°C for 30 minutes. In ELISA and otherimmunoreactive assays, this antiserum will recognize both native and recombinant humanGM-CSF in cell supernatants and certain body fluids. This antibody is useful forneutralization of human GM-CSF in bioassays. For Neutralization, it is recommended to incubate the sample with a 1: 400 dilution of theantiserum for at least 4 hours before being tested. A control of similarly diluted normalRabbit IgG is recommended. Add. Information: Endotoxin Content: < 10 pg/ml by LAL method.

Application Details

Application Notes: This antiserum against Human GM-CSF has been tested for use in Neutralizations, ELISA,Radioimmunoassays, Immunohistochemistry and Immunoblotting. Reactivity in otherimmunoassays is unknown. Recommended Dilutions: This product has been assayed by immunoblot using HRPGoat-anti-Rabbit IgG [H&L] and TMB as a substrate. A working dilution of 1: 400 issuggested for this application. This product has been assayed by Immunohistochemistry. A dilution of 1: 400 or greater is suggested for this immunoassay. Either paraffin fixation orcryofixation can be used for Immunohistochemistry. This product has been assayed byELISA against GM-CSF using HRP Conjugated Anti-Rabbit IgG [H&L] (Goat) and ABTS as asubstrate for 30 minutes at room temperature. A working dilution range of 1: 600 to 1: 1,200is suggested for this product. For use in ELISA formats, this antibody is best used as thesecond antibody in combination with a monoclonal antibody as a capture antibody. Thisproduct has been assayed by Radioimmunoassay against antigen. A dilution of 1: 8,000 issuggested for this immunoassay. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.

Concentration: 85 mg/ml (by Refractometry)

Storage: Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Dilute only prior to immediate use. Avoid cycles of freezing and thawing. Shelf life: One year from despatch.

Research Area: Immunology, Innate Immunity, Cytokines

Restrictions: For Research Use only