Interleukin 1 Receptor, Type I (IL1R1) antibody

Details for Product No. ABIN116871
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Antigen
Synonyms CD121A, D2S1473, IL-1R-alpha, IL1R, IL1RA, P80, CD121a, CD121b, IL-iR, Il1r-1
Reactivity
Human
(62), (29), (13)
Host
Rabbit
(54), (21), (17), (10), (1), (1)
Clonality
Polyclonal
Conjugate
Un-conjugated
(5), (2), (1), (1), (1), (1), (1)
Application
Enzyme Immunoassay (EIA), Immunoprecipitation (IP)
(61), (60), (19), (16), (15), (14), (14), (10), (8), (6), (5), (3), (2), (2), (2), (2)
Pubmed 10 references available
Quantity 1 mL
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Catalog No. ABIN116871
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Immunogen The whole rabbit serum was prepared by repeated immunizations with recombinant human IL-1R type I.
Specificity This antiserum has been heated to 56 °C for 30 minutes. The antiserum is directed against the extracellular domain of the human IL-1R type I, but precipitates the intact receptor expressed on human cells. In general, this antibody also detects primate IL-1RI. The antibody does not recognize human IL-1, IL-1Ra or IL-1R type II. In ELISA formats and other immunoreactive assays, this antibody will detect the extracellular domain of the IL-1R type I (also called soluble IL-1R type I or "decoy" receptor) found in human body fluids, particularly in the circulation, urine and supernatants of cells and synovial fluid.
Purification Serum
Alternative Name CD121a / IL1R1
Background IL1 binds antigen CD121a, which is a transmembrane glycoprotein and a member of the immunoglobulin superfamily expressed on T cells, thymocytes, dendritic cells, fibroblasts, vascular endothelial cells, epithelial cells and neural cells. CD121a binds the mature forms of IL1 alpha, IL1 beta, and IL1 Ra proteins. High affinity binding and signalling by the type I IL1 receptor requires CD121a and the accessory protein, IL1 R AcP, which does not bind IL1 alone, but forms a dimer capable of binding IL1. Interleukin 1 (IL1), which has a role as a mediator in inflammation, consists of 2 separate but related proteins, IL1 alpha and IL1 beta. Cell surface receptors for the 2 forms of IL1 are identical. IL1R Type I contains a single membrane spanning segment, a large cytoplasmic region, and an extracellular, IL1 binding portion composed of 3 immunoglobulin like domains.Synonyms: IL-1R-alpha, IL1R, IL1RA, IL1RT1, Interleukin-1 receptor alpha, Interleukin-1 receptor type 1, Interleukin-1 receptor type I
Gene ID 3554
NCBI Accession NP_000868
UniProt P14778
Research Area CD Antigens, Surface Receptors of Immune Cells
Application Notes This antiserum against anti-Human IL-1 Receptor Type I (IL-1RI) has been tested for use inELISA, Radioimmunoassays and Immunoprecipitation. Reactivity in other immunoassays isunknown. Recommended Dilutions: This product has been assayed for the ability toimmunoprecipitate antigen. A dilution range of 1: 400 to 1: 800 is suggested for thisimmunoassay. For immunoprecipitation, pre-clearing with a non-specific rabbit IgG ishelpful to reduce background. This product has been assayed by ELISA against soluble(extracellular) IL-1RI using HRP Conjugated Anti-Rabbit IgG [H&L] (Goat) (R1364HRP) andABTS as a substrate for 30 minutes at room temperature. A working dilution range of1: 1,000 to 1: 2000 is suggested for this product. This product has been assayed byradioimmunoassay against antigen. A dilution of 1: 8,000 is suggested for thisimmunoassay.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Format Liquid
Concentration 85 mg/mL (by Refractometry)
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store lyophilized product at 2-8 °C. Store the reconstituted antibody at 2-8 °C for one month (add 0.09% Sodium Azide) or at -20 °C for longer.
Background publications Mann, Micouin, Chiannilkulchai et al.: "RPC53 encodes a subunit of Saccharomyces cerevisiae RNA polymerase C (III) whose inactivation leads to a predominantly G1 arrest." in: Molecular and cellular biology, Vol. 12, Issue 10, pp. 4314-26, 1992 (PubMed).

McMahan, Slack, Mosley et al.: "A novel IL-1 receptor, cloned from B cells by mammalian expression, is expressed in many cell types." in: The EMBO journal, Vol. 10, Issue 10, pp. 2821-32, 1991 (PubMed).

Dower, Wignall, Schooley et al.: "Retention of ligand binding activity by the extracellular domain of the IL-1 receptor." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 142, Issue 12, pp. 4314-20, 1989 (PubMed).

Zagorski, Tollervey, Fournier: "Characterization of an SNR gene locus in Saccharomyces cerevisiae that specifies both dispensible and essential small nuclear RNAs." in: Molecular and cellular biology, Vol. 8, Issue 8, pp. 3282-90, 1989 (PubMed).

Colotta, Dower, Sims et al.: "The type II 'decoy' receptor: a novel regulatory pathway for interleukin 1." in: Immunology today, Vol. 15, Issue 12, pp. 562-6, 1995 (PubMed).

Arend, Malyak, Smith et al.: "Binding of IL-1 alpha, IL-1 beta, and IL-1 receptor antagonist by soluble IL-1 receptors and levels of soluble IL-1 receptors in synovial fluids." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 153, Issue 10, pp. 4766-74, 1994 (PubMed).

Vigers, Caffes, Evans et al.: "X-ray structure of interleukin-1 receptor antagonist at 2.0-A resolution." in: The Journal of biological chemistry, Vol. 269, Issue 17, pp. 12874-9, 1994 (PubMed).

Colotta, Re, Muzio et al.: "Interleukin-1 type II receptor: a decoy target for IL-1 that is regulated by IL-4." in: Science (New York, N.Y.), Vol. 261, Issue 5120, pp. 472-5, 1993 (PubMed).

Pruitt, Welborn, Edwards et al.: "Increased soluble interleukin-1 type II receptor concentrations in postoperative patients and in patients with sepsis syndrome." in: Blood, Vol. 87, Issue 8, pp. 3282-8, 1996 (PubMed).

Schreuder, Tardif, Trump-Kallmeyer et al.: "A new cytokine-receptor binding mode revealed by the crystal structure of the IL-1 receptor with an antagonist." in: Nature, Vol. 386, Issue 6621, pp. 194-200, 1997 (PubMed).

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