Interleukin 1 Receptor, Type I (IL1R1) antibody

Antigen: Interleukin 1 Receptor, Type I (IL1R1)

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Conjugate: Un-conjugated

Application: Immunoprecipitation (IP), ELISA

Catalog no.: ABIN116871

Additional Information

Alternative name: CD121a / IL1R1

UniProt: P14778

Immunogen: The whole rabbit serum was prepared by repeated immunizations with recombinant humanIL-1R type I.

Cross-Reactivity: Human

Description: IL1 binds antigen CD121a, which is a transmembrane glycoprotein and a member of theimmunoglobulin superfamily expressed on T cells, thymocytes, dendritic cells, fibroblasts,vascular endothelial cells, epithelial cells and neural cells. CD121a binds the mature formsof IL1 alpha, IL1 beta, and IL1 Ra proteins. High affinity binding and signalling by the type IIL1 receptor requires CD121a and the accessory protein, IL1 R AcP, which does not bind IL1alone, but forms a dimer capable of binding IL1. Interleukin 1 (IL1), which has a role as amediator in inflammation, consists of 2 separate but related proteins, IL1 alpha and IL1beta. Cell surface receptors for the 2 forms of IL1 are identical. IL1R Type I contains a singlemembrane spanning segment, a large cytoplasmic region, and an extracellular, IL1 bindingportion composed of 3 immunoglobulin like domains.

Characteristics: Synonyms: Interleukin-1 receptor type I, IL-1R-1, IL-1RT1, IL-1R-alpha, p80, IL1RA, IL1RT1, CD121antigen-like family member A

Specificity: This antiserum has been heated to 56°C for 30 minutes. The antiserum is directed againstthe extracellular domain of the human IL-1R type I, but precipitates the intact receptorexpressed on human cells. In general, this antibody also detects primate IL-1RI. The antibody does not recognize human IL-1, IL-1Ra or IL-1R type II. In ELISA formats andother immunoreactive assays, this antibody will detect the extracellular domain of the IL-1Rtype I (also called soluble IL-1R type I or decoy receptor) found in human body fluids,particularly in the circulation, urine and supernatants of cells and synovial fluid. Add. Information: Endotoxin Content: < 10 pg/µl by LAL method.

Application Details

Application Notes: This antiserum against anti-Human IL-1 Receptor Type I (IL-1RI) has been tested for use inELISA, Radioimmunoassays and Immunoprecipitation. Reactivity in other immunoassays isunknown. Recommended Dilutions: This product has been assayed for the ability toimmunoprecipitate antigen. A dilution range of 1: 400 to 1: 800 is suggested for thisimmunoassay. For immunoprecipitation, pre-clearing with a non-specific rabbit IgG ishelpful to reduce background. This product has been assayed by ELISA against soluble(extracellular) IL-1RI using HRP Conjugated Anti-Rabbit IgG [H&L] (Goat) (R1364HRP) andABTS as a substrate for 30 minutes at room temperature. A working dilution range of1: 1,000 to 1: 2000 is suggested for this product. This product has been assayed byradioimmunoassay against antigen. A dilution of 1: 8,000 is suggested for thisimmunoassay. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.

Concentration: 85 mg/ml (by Refractometry)

Purification: Serum

Storage: Store the antibody (undiluted) at 2-8°C for one month or (in aliquots) at -20°C for longer. Dilute only prior to immediate use. Avoid cycles of freezing and thawing. Shelf life: One year from despatch.

Research Area: CD Antigens, Surface Receptors of Immune Cells

Restrictions: For Research Use only

Publications

Colotta, Dower, Sims et al.: "The type II 'decoy' receptor: a novel regulatory pathway for interleukin 1." in: Immunology today, Vol. 15, Issue 12, pp. 562-6, 1995 (PubMed).

Arend, Malyak, Smith et al.: "Binding of IL-1 alpha, IL-1 beta, and IL-1 receptor antagonist by soluble IL-1 receptors and levels of soluble IL-1 receptors in synovial fluids." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 153, Issue 10, pp. 4766-74, 1994 (PubMed).

Vigers, Caffes, Evans et al.: "X-ray structure of interleukin-1 receptor antagonist at 2.0-A resolution." in: The Journal of biological chemistry, Vol. 269, Issue 17, pp. 12874-9, 1994 (PubMed).

Colotta, Re, Muzio et al.: "Interleukin-1 type II receptor: a decoy target for IL-1 that is regulated by IL-4." in: Science (New York, N.Y.), Vol. 261, Issue 5120, pp. 472-5, 1993 (PubMed).

Schreuder, Tardif, Trump-Kallmeyer et al.: "A new cytokine-receptor binding mode revealed by the crystal structure of the IL-1 receptor with an antagonist." in: Nature, Vol. 386, Issue 6621, pp. 194-200, 1997 (PubMed).