Western Blotting (WB), Immunoprecipitation (IP), Enzyme Immunoassay (EIA)
Specificity
Anti-IKKa may react non-specifically with other proteins. Control peptide will compete only with the specific reaction of antiserum with the IKKa subunit.
Purification
Prepared from monospecific antiserum by delipidation and defibrination.
Immunogen
IKKa peptide corresponding to the highly conserved C-terminus region of the human protein conjugated to Keyhole Limpet Hemocyanin (KLH).
Suitable for Immunoprecipitation, Immunoblotting and ELISA. Recommended Dilutions: This product was assayed by immunoblot and found to bereactive against IKKa at a dilution of 1: 1000 followed by reaction with Peroxidaseconjugated Affinity Purified anti-Rabbit IgG [H&L] (Goat). Anti-IKKa is suitable for thedetection by immunoblot of human, mouse and rat IKKa showing an 85 kDa band. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions
For Research Use only
Format
Liquid
Concentration
80.0 mg/mL (by Refractometry)
Handling Advice
Avoid repeated freezing and thawing.
Storage
4 °C/-20 °C
Storage Comment
Store lyophilized product at 2-8 °C. After reconstitution, store at -20 °C.
NFk B comprises a family of cellular transcription factors that are involved in the inducible expression of a variety of cellular genes that regulate the inflammatory response and control of cell death. In the cytoplasm NFk B is negatively modulated by the inhibitory proteins Ik B. In turn Ik B is phosphorylated by a cellular kinase complex called IKK. IKK is a heterodimer composed of two kinases: IKK-a and IKK-b that phosphorylate Ik B leading to its degradation and the resulting translocation of NFk B to the nucleus. IKK kinase activity is modulated negatively by pharmaceutical agents such as aspirin and positively by various cellular components such as TNF-a , endotoxins and overexpression of cellular kinases like MEKK1. Aspirin appears to have its effect by inhibiting the binding of ATP to IKK.Synonyms: CHUK, Conserved helix-loop-helix ubiquitous kinase, I kappa-B kinase alpha, I-kappa-B kinase 1, IKK-A, IKK-alpha, IKK1, IkBKA, IkappaB kinase, Inhibitor of nuclear factor kappa-B kinase subunit alpha, NFKBIKA, Nuclear factor NF-kappa-B inhibitor kinase alpha, TCF16