Fructosyl Amino Acid Oxidase (socD) antibody (Biotin)

Antigen: Fructosyl Amino Acid Oxidase (socD)

Clonality: Polyclonal

Host: Sheep

Conjugate: Biotin

Application: Enzyme Immunoassay (EIA)

Catalog no.: ABIN116881

Additional Information

Alternative name: Fructosyl-Amino Acid Oxidase [E.coli]

Immunogen: Fructosyl Amino Acid Oxidase [E. coli].

Description: Fructosyl amino acid oxidase (FAOX or FAOD) catalyzes the oxidative deglycation offructosyl amino acids, yielding the corresponding amino acid, glucosone, and hydrogenperoxide. Glucose can be attached to aminoacids by a nonenzymatic reaction calledglycation, to distinguish it from the enzymatic glycosylation of proteins. (FAOX) can be used for the enzymatic detection of nonenzymatically glycated proteins. Glycation of protein has been implicated in the development of diabetic complications andthe aging process. Glycation of blood proteins such as hemoglobin and albumin isenhanced in diabetics with high blood glucose. The amounts of these glycated proteinsreflect the level of blood glucose in periods corresponding to the half life of the protein (14to 20 days for albumin and 1 to 2 months for hemoglobin). Since the glycation of bloodproteins is not affected by transient increases in blood glucose, the levels of glycatedproteins are good indices for monitoring diabetes mellitus patients during therapy. HbA1c,blood levels of which are a good index of the long term control of blood glucose in patientswith diabetes mellitus, is defined as an amino end of the ß subunit (valine residue) ofhemoglobin that has been glycated nonenzymatically.

Specificity: This product is an IgG fraction antibody purified from monospecific antiserum by amultistep process which includes delipidation, salt fractionation and ion exchangechromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Biotin,anti-Sheep Serum as well as purified and partially purified Fructosyl Amino Acid Oxidase[E. coli]. Cross reactivity against Fructosyl Amino Acid Oxidase from other sources is unknown.

Application Details

Application Notes: Suitable for Immunoblotting, ELISA, Immunohistochemistry, Immunomicroscopy as well asother antibody based assays using streptavidin or avidin conjugates requiring lot-to-lotconsistency. Recommended Diutions: This product has been assayed against 1.0 g of Fructosyl AminoAcid Oxidase in a standard capture ELISA using Peroxidase Conjugated Streptavidin andABTS (2,2'-azino-bis-[3- ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutesat room temperature. A working dilution of 1: 8,000 to 1: 34,000 of the reconstitutionconcentration is suggested for this product.

Concentration: 10.0 mg/ml (by UV absorbance at 280 nm)

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 with 10mg/ml Bovine Serum Albumin (BSA, IgG and Protease free) as stabilizer and 0.01% (w/v)Sodium Azide as preservative.Label: Biotinamidocaproate N-Hydroxysuccinimide Ester (BAC). Molar Ratio:

Storage: Store vial at 2-8°C prior to restoration. For extended storage add glycerol to 50% and thenaliquot contents and freeze at -20°C or below. Centrifuge product if not completely clearafter standing at room temperature. This antibody is stable for one month at 2-8°C as an undiluted liquid. Dilute only prior to immediate use. Avoid repeated freezing and thawing. Shelf life: One year from despatch.

Research Area: Metabolism

Restrictions: For Research Use only

Publications

Publications: Wilchek, Bayer: "Labeling glycoconjugates with hydrazide reagents." in: Methods in enzymology, Vol. 138, pp. 429-42, 1987 (PubMed).