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Recombinant alpha Tubulin antibody

TUBA1 Reactivity: Human, Mouse, Cow WB, ICC Host: Human Monoclonal F2C unconjugated Recombinant Antibody
Catalog No. ABIN1169084
  • Target See all alpha Tubulin (TUBA1) Antibodies
    alpha Tubulin (TUBA1)
    Antibody Type
    Recombinant Antibody
    Reactivity
    • 108
    • 73
    • 67
    • 24
    • 18
    • 16
    • 14
    • 13
    • 8
    • 5
    • 5
    • 4
    • 4
    • 4
    • 4
    • 4
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Human, Mouse, Cow
    Host
    • 62
    • 55
    • 8
    • 2
    • 1
    • 1
    • 1
    Human
    Clonality
    • 71
    • 56
    • 2
    Monoclonal
    Conjugate
    • 84
    • 11
    • 6
    • 5
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    This alpha Tubulin antibody is un-conjugated
    Application
    • 102
    • 45
    • 42
    • 42
    • 38
    • 33
    • 27
    • 14
    • 7
    • 6
    • 4
    • 2
    • 1
    • 1
    • 1
    • 1
    Western Blotting (WB), Immunocytochemistry (ICC)
    Specificity
    Recognizes mouse, bovine and human alpha-tubulin.
    Cross-Reactivity
    Cow (Bovine), Human, Mouse (Murine)
    Purification
    Purified from HEK 293 cell culture supernatant.
    Purity
    >95 % (SDS-PAGE)
    Immunogen
    Bovine brain tubulin.
    Clone
    F2C
    Isotype
    IgG2
    Top Product
    Discover our top product TUBA1 Primary Antibody
  • Application Notes
    Optimal working dilution should be determined by the investigator.
    Comment

    anti-alpha-Tubulin, monoclonal antibody (recombinant) (F2C) is composed of human variable regions (VH and VL) (lambda-chain) of immunoglobulin fused to the human lgG2 Fc domain. anti-alpha-Tubulin, monoclonal antibody (recombinant) (F2C) is an antibody developed by antibody phage display technology using a human naive antibody gene library. These libraries consist of scFv (single chain fragment variable) composed of VH (variable domain of the human immunoglobulin heavy chain) and VL (variable domain of the human immunoglobulin light chain) connected by a polypeptide linker. The antibody fragments are displayed on the surface of filamentous bacteriophage (M13). This scFv was selected by affinity selection on antigen in a process termed panning. Multiple rounds of panning are performed to enrich for antigen-specific scFv-phage. Monoclonal antibodies are subsequently identified by screening after each round of selection. The selected monoclonal scFv is cloned into an appropriate vector containing a Fc portion of interest and then produced in mammalian cells to generate an IgG like scFv-Fc fusion protein.

    Restrictions
    For Research Use only
  • Format
    Liquid
    Concentration
    Lot specific
    Buffer
    In PBS containing 10 % glycerol and 0.02 % sodium azide.
    Preservative
    Sodium azide
    Precaution of Use
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Storage
    4 °C,-20 °C
    Storage Comment
    Short Term Storage: +4°C
    Long Term Storage: -20°C
    Stable for at least 1 month after receipt when stored at +4°C. Stable for at least 1 year after receipt when stored at -20°C.
    Expiry Date
    12 months
  • Vielemeyer, Nizak, Jimenez, Echard, Goud, Camonis, Rain, Perez: "Characterization of single chain antibody targets through yeast two hybrid." in: BMC biotechnology, Vol. 10, pp. 59, (2010) (PubMed).

    Nizak, Martin-Lluesma, Moutel, Roux, Kreis, Goud, Perez: "Recombinant antibodies against subcellular fractions used to track endogenous Golgi protein dynamics in vivo." in: Traffic (Copenhagen, Denmark), Vol. 4, Issue 11, pp. 739-53, (2003) (PubMed).

  • Target
    alpha Tubulin (TUBA1)
    Alternative Name
    alpha-Tubulin (TUBA1 Products)
    Synonyms
    Tuba1 antibody, K-ALPHA-1 antibody, Tuba-1 antibody, cb944 antibody, fb22g06 antibody, tuba1 antibody, wu:fb22g06 antibody, Calpha1 antibody, tubulin, alpha 1A antibody, tubulin alpha 1b antibody, tubulin, alpha 1b antibody, tubulin, alpha 1B antibody, Tuba1a antibody, TUBA1B antibody, tuba1b antibody, Tuba1b antibody
    Background
    Tubulin is the major building block of microtubules. This intracellular cylindrical filamentous structure is present in almost all eukaryotic cells. Microtubules function as structural and mobile elements in mitosis, intracellular transport, flagellar movement, and the cytoskeleton.
    Pathways
    Microtubule Dynamics
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