alpha Tubulin antibody (TUBA1)

Details for Product anti-TUBA1 Antibody No. ABIN1169084, Supplier: Log in to see
Antigen
  • K-ALPHA-1
  • TUBA2
  • mec-12
  • tuba4
  • alpha tubulin
  • similar to alpha tubulin
  • tubulin-alpha
  • tubulin, alpha 1b
  • tubulin, alpha 3e
  • tubulin, alpha 4b
  • LOC100009327
  • RGD1560000
  • LOC100101229
  • TUBA1B
  • TUBA3E
  • tuba4b
Alternatives
anti-Human alpha Tubulin antibody for Western Blotting
Reactivity
Cow (Bovine), Human, Mouse (Murine)
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Host
Human
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Antibody Type
Recombinant Antibody
Clonality (Clone)
Monoclonal ()
Conjugate
This alpha Tubulin antibody is un-conjugated
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Application
Immunocytochemistry (ICC), Western Blotting (WB)
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Options
Supplier
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Supplier Product No.
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Immunogen Bovine brain tubulin.
Clone F2C
Isotype IgG2
Specificity Recognizes mouse, bovine and human alpha-tubulin.
Cross-Reactivity Cow (Bovine), Human, Mouse (Murine)
Purification Purified from HEK 293 cell culture supernatant.
Purity >95 % (SDS-PAGE)
Alternative Name alpha-Tubulin (TUBA1 Antibody Abstract)
Background Tubulin is the major building block of microtubules. This intracellular cylindrical filamentous structure is present in almost all eukaryotic cells. Microtubules function as structural and mobile elements in mitosis, intracellular transport, flagellar movement, and the cytoskeleton.
Research Area Cytoskeleton
Pathways Microtubule Dynamics
Application Notes Optimal working dilution should be determined by the investigator.
Comment

anti-alpha-Tubulin, monoclonal antibody (recombinant) (F2C) is composed of human variable regions (VH and VL) (lambda-chain) of immunoglobulin fused to the human lgG2 Fc domain. anti-alpha-Tubulin, monoclonal antibody (recombinant) (F2C) is an antibody developed by antibody phage display technology using a human naive antibody gene library. These libraries consist of scFv (single chain fragment variable) composed of VH (variable domain of the human immunoglobulin heavy chain) and VL (variable domain of the human immunoglobulin light chain) connected by a polypeptide linker. The antibody fragments are displayed on the surface of filamentous bacteriophage (M13). This scFv was selected by affinity selection on antigen in a process termed panning. Multiple rounds of panning are performed to enrich for antigen-specific scFv-phage. Monoclonal antibodies are subsequently identified by screening after each round of selection. The selected monoclonal scFv is cloned into an appropriate vector containing a Fc portion of interest and then produced in mammalian cells to generate an IgG like scFv-Fc fusion protein.

Restrictions For Research Use only
Format Liquid
Concentration 1 mg/mL
Buffer In PBS containing 10 % glycerol and 0.02 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C,-20 °C
Storage Comment Short Term Storage: +4°C
Long Term Storage: -20°C
Stable for at least 1 month after receipt when stored at +4°C. Stable for at least 1 year after receipt when stored at -20°C.
Expiry Date 12 months
Product cited in: Vielemeyer, Nizak, Jimenez, Echard, Goud, Camonis, Rain, Perez: "Characterization of single chain antibody targets through yeast two hybrid." in: BMC biotechnology, Vol. 10, pp. 59, 2010 (PubMed).

Nizak, Martin-Lluesma, Moutel, Roux, Kreis, Goud, Perez: "Recombinant antibodies against subcellular fractions used to track endogenous Golgi protein dynamics in vivo." in: Traffic (Copenhagen, Denmark), Vol. 4, Issue 11, pp. 739-53, 2003 (PubMed).

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