BTLA antibody

Catalog No. ABIN1169098

Product Details anti-BTLA Antibody

Target: BTLA (B and T Lymphocyte Associated (BTLA))

Reactivity: Human

Host: Rat

Clonality: Monoclonal

Conjugate: This BTLA antibody is un-conjugated

Application: ELISA, Flow Cytometry (FACS)

Specificity: Recognizes human BTLA.

Cross-Reactivity: Human

Purification: Purified from concentrated hybridoma tissue culture supernatant.

Purity: >95 % (SDS-PAGE)

Endotoxin Level: <0.001EU/μg purified protein.

Immunogen: Ectodomain of human BTLA fused to human IgG Fc.

Clone: 6F4

Isotype: IgG1

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: In PBS containing 10 % glycerol.

Storage: 4 °C,-20 °C

Storage Comment: Short Term Storage: +4°C
Long Term Storage: -20°C
Stable for at least 1 year after receipt when stored at -20°C.

Expiry Date: 12 months

References for anti-BTLA antibody (ABIN1169098)

Cheung, Oborne, Steinberg, Macauley, Fukuyama, Sanjo, DSouza, Norris, Pfeffer, Murphy, Kronenberg, Spear, Ware: "T cell intrinsic heterodimeric complexes between HVEM and BTLA determine receptivity to the surrounding microenvironment." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 183, Issue 11, pp. 7286-96, (2009) (PubMed).

Cheung, Steinberg, Oborne, Macauley, Fukuyama, Sanjo, DSouza, Norris, Pfeffer, Murphy, Kronenberg, Spear, Ware: "Unconventional ligand activation of herpesvirus entry mediator signals cell survival." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 106, Issue 15, pp. 6244-9, (2009) (PubMed).

Target Details for BTLA

Target: BTLA (B and T Lymphocyte Associated (BTLA))

Alternative Name: BTLA (BTLA Products)

Synonyms: BTLA antibody, BTLA1 antibody, CD272 antibody, A630002H24 antibody, B and T lymphocyte associated antibody, BTLA antibody, Btla antibody

Background: B and T lymphocyte associated (BTLA) is an Ig domain superfamily protein with cytoplasmic immunoreceptor tyrosine-based inhibitory motifs. The herpes virus entry mediator (HVEM), a member of the TNF receptor (TNFR) superfamily, can act as a molecular switch that modulates T cell activation by propagating positive signals from the TNF-related ligand LIGHT (TNFR superfamily 14), or inhibitory signals through the Ig superfamily member BTLA. The binding site on HVEM for BTLA is conserved in the orphan TNF receptor UL144, present in human CMV. UL144 binds BTLA, but not LIGHT, and inhibits T cell proliferation, selectively mimicking the inhibitory cosignaling function of HVEM.

UniProt: Q7Z6A9

Pathways: Cancer Immune Checkpoints