Rab1-GTP antibody

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Antigen
Reactivity
Dog (Canine), Human, Mouse (Murine), Rat (Rattus)
2
1
1
1
Host
Human
1
1
Antibody Type
Recombinant Antibody
Clonality (Clone)
Monoclonal ()
Application
Immunocytochemistry (ICC), Immunoprecipitation (IP)
2
1
1
Options
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Immunogen Full length canine Rab1.
Clone ROF7
Isotype IgG2
Fragment lambda
Specificity Recognizes human, mouse, rat and dog Rab1a-GTP and Rab1b-GTP.
Cross-Reactivity Dog (Canine), Human, Mouse (Murine), Rat (Rattus)
Purification Purified from HEK 293 cell culture supernatant.
Purity >95 % (SDS-PAGE)
Background Rab1 (Ypt1 in yeast) is a small GTPase that plays a well-established role in mediating ER-to-Golgi protein transport in both yeast and mammalian cells. Rab1 recruits effector proteins to budding COPII vesicles at the ER, forming cis-SNARE complexes that promote targeting to and fusion of these vesicles with the cis-Golgi. Rab1 is also involved in COPI vesicle formation and other distinct transport pathways, including ER-to-Golgi intermediate compartment (ERGIC)-to-cell periphery trafficking. Recently Rab1 has been shown to function in antimicrobial autophagy (autophagosome formation), as well as other forms of autophagy in mammalian cells in a way independent of ER-to-Golgi trafficking. anti-Rab1-GTP, monoclonal antibody (recombinant) (ROF7) is an antibody developed by antibody phage display technology using a human naive antibody gene library. These libraries consist of scFv (single chain fragment variable) composed of VH (variable domain of the human immunoglobulin heavy chain) and VL (variable domain of the human immunoglobulin light chain) connected by a polypeptide linker. The antibody fragments are displayed on the surface of filamentous bacteriophage (M13). This scFv was selected by affinity selection on antigen in a process termed panning. Multiple rounds of panning are performed to enrich for antigen-specific scFv-phage. Monoclonal antibodies are subsequently identified by screening after each round of selection. The selected monoclonal scFv is cloned into an appropriate vector containing a Fc portion of interest and then produced in mammalian cells to generate an IgG like scFv-Fc fusion protein.
UniProt P62822
Application Notes Optimal working dilution should be determined by the investigator.
Comment

anti-Rab1-GTP, monoclonal antibody (recombinant) (ROF7) is composed of human variable regions (VH and VL) (lambda-chain) of immunoglobulin fused to the human lgG2 Fc domain.

Restrictions For Research Use only
Format Liquid
Concentration 1 mg/mL
Buffer In PBS containing 10 % glycerol and 0.02 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C,-20 °C
Storage Comment Short Term Storage: +4°C
Long Term Storage: -20°C
Stable for at least 1 month after receipt when stored at +4°C. Stable for at least 1 year after receipt when stored at -20°C.
Expiry Date 12 months
Product cited in: Vielemeyer, Nizak, Jimenez, Echard, Goud, Camonis, Rain, Perez: "Characterization of single chain antibody targets through yeast two hybrid." in: BMC biotechnology, Vol. 10, pp. 59, 2010 (PubMed).