GLN1 antibody

Catalog No. ABIN116921

Product Details anti-GLN1 Antibody

Target: GLN1 (Glutamine Synthetase (GLN1))

Reactivity: Bacillus

Host: Goat

Clonality: Polyclonal

Conjugate: This GLN1 antibody is un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP), Enzyme Immunoassay (EIA)

Specificity: This product was prepared from monospecific antiserum by a delipidation and defibrination. Assay by immunoelectrophoresis resulted in a single precipitin arc against purified and partially purified Glutamine Synthetase [Microbial]. Cross reactivity against Glutamine Synthetase from other sources is unknown.

Purification: Delipidation and Defibrination.

Immunogen: Glutamine Synthetase [Microbial].

Application Details

Application Notes: Suitable for Immunoblotting (Western or Dot blot), ELISA, Immunoprecipitation and mostimmunological methods requiring high titer and specificity. Recommended Dilutions: This product has been assayed against 1.0 μg of GlutamineSynthetase [Microbial] in a standard sandwich ELISA using Peroxidase conjugated AffinityPurified anti-Goat IgG [H&L] (Rabbit) and (ABTS(2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes atroom temperature. A working dilution of 1: 5,000 to 1: 50,000 of the reconstitutionconcentration is suggested for this product.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Format: Liquid

Reconstitution: Restore with 2.0 mL of deionized water (or equivalent).

Concentration: 90.0 mg/mL (by Refractometry)

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 with 0.01 % (w/v) Sodium Azide as preservative.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing. Dilute only prior to immediate use

Storage: 4 °C/-20 °C

Storage Comment: Store vial at 2-8 °C prior to restoration. For extended storage add glycerol to 50% and then aliquot contents and freeze at -20 °C or below. Centrifuge product if not completely clear after standing at room temperature. This antibody is stable for one month at 2-8 °C as an undiluted liquid.

Target Details for GLN1

Target: GLN1 (Glutamine Synthetase (GLN1))

Alternative Name: Glutamine Synthetase (GLN1 Products)

Synonyms: GLNS antibody, GS antibody, PIG43 antibody, PIG59 antibody, Glns antibody, glutamate-ammonia ligase antibody, glutamate-ammonia ligase (glutamine synthetase) antibody, glutamine synthetase antibody, GLUL antibody, Glul antibody, GLN1 antibody

Background: Synonyms: GLNS, GLUL, GS, Glutamate decarboxylase, Glutamate-ammonia ligase

Gene ID: 948370

NCBI Accession: AP_003938

UniProt: P0A9C5

Pathways: Positive Regulation of Peptide Hormone Secretion