DDX58 antibody (AA 201-713)

Catalog No. ABIN1169336

Product Details anti-DDX58 Antibody

Target: DDX58 (DEAD (Asp-Glu-Ala-Asp) Box Polypeptide 58 (DDX58))

Binding Specificity: AA 201-713

Reactivity: Human, Mouse

Host: Mouse

Clonality: Monoclonal

Conjugate: This DDX58 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP)

Specificity: Recognizes human and mouse RIG-I.

Cross-Reactivity: Human, Mouse (Murine)

Purification: Purified from concentrated hybridoma tissue culture supernatant.

Purity: >95 % (SDS-PAGE)

Immunogen: Recombinant human RIG-I (aa 201-713).

Clone: Alme-1

Isotype: IgG1

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: In PBS containing 10 % glycerol and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Short Term Storage: +4°C
Long Term Storage: -20°C
Stable for at least 1 year after receipt when stored at -20°C.

Expiry Date: 12 months

References for anti-DDX58 antibody (ABIN1169336)

Kell, Hemann, Turnbull, Gale: "RIG-I-like receptor activation drives type I IFN and antiviral signaling to limit Hantaan orthohantavirus replication." in: PLoS pathogens, Vol. 16, Issue 4, pp. e1008483, (2020) (PubMed).

Moriyama, Koshiba, Ichinohe: "Influenza A virus M2 protein triggers mitochondrial DNA-mediated antiviral immune responses." in: Nature communications, Vol. 10, Issue 1, pp. 4624, (2020) (PubMed).

Soonthornvacharin, Rodriguez-Frandsen, Zhou, Galvez, Huffmaster, Tripathi, Balasubramaniam, Inoue, de Castro, Moulton, Stein, Sánchez-Aparicio, De Jesus, Nguyen, König, Krogan, García-Sastre, Yoh et al.: "Systems-based analysis of RIG-I-dependent signalling identifies KHSRP as an inhibitor of RIG-I receptor activation. ..." in: Nature microbiology, Vol. 2, pp. 17022, (2018) (PubMed).

Harden, Munger: "Human papillomavirus 16 E6 and E7 oncoprotein expression alters microRNA expression in extracellular vesicles." in: Virology, Vol. 508, pp. 63-69, (2017) (PubMed).

Pythoud, Rothenberger, Martínez-Sobrido, de la Torre, Kunz: "Lymphocytic Choriomeningitis Virus Differentially Affects the Virus-Induced Type I Interferon Response and Mitochondrial Apoptosis Mediated by RIG-I/MAVS." in: Journal of virology, Vol. 89, Issue 12, pp. 6240-50, (2015) (PubMed).

Weber, Gawanbacht, Habjan, Rang, Borner, Schmidt, Veitinger, Jacob, Devignot, Kochs, García-Sastre, Weber: "Incoming RNA virus nucleocapsids containing a 5'-triphosphorylated genome activate RIG-I and antiviral signaling." in: Cell host & microbe, Vol. 13, Issue 3, pp. 336-46, (2013) (PubMed).

Gack, Nistal-Villán, Inn, García-Sastre, Jung: "Phosphorylation-mediated negative regulation of RIG-I antiviral activity." in: Journal of virology, Vol. 84, Issue 7, pp. 3220-9, (2010) (PubMed).

Rebsamen, Meylan, Curran, Tschopp: "The antiviral adaptor proteins Cardif and Trif are processed and inactivated by caspases." in: Cell death and differentiation, Vol. 15, Issue 11, pp. 1804-11, (2008) (PubMed).

Target Details for DDX58

Target: DDX58 (DEAD (Asp-Glu-Ala-Asp) Box Polypeptide 58 (DDX58))

Alternative Name: RIG-I (DDX58 Products)

Synonyms: RIG-I antibody, RIGI antibody, RLR-1 antibody, 6430573D20Rik antibody, C330021E21 antibody, RHIV-1 antibody, RIG-1 antibody, DExD/H-box helicase 58 antibody, DEAD (Asp-Glu-Ala-Asp) box polypeptide 58 antibody, DEXD/H-box helicase 58 antibody, DDX58 antibody, Ddx58 antibody

Background: RIG-I and MDA5 are highly conserved helicases involved in the innate immune response to virus. RIG-I is a member of the DEAD-box RNA helicases and is activated by cytoplasmic dsRNA and 5'-ppp RNA produced during the viral replication. The protein is characterized by a N-terminal region with two caspase recruitment domains (CARD) and a C-terminal region harboring potential ATP-dependent RNA helicase activity. RIG-I recruits the CARD adaptor inducing IFN-beta (Cardif) in a CARD-CARD-dependent manner resulting in NF-kappaB and IRF3 activation.

UniProt: O95786

Pathways: Activation of Innate immune Response, Hepatitis C