IKKi/IKKe antibody (pThr501)

Catalog No. ABIN116937

Product Details anti-IKKi/IKKe Antibody

Target: IKKi/IKKe (IKBKE) (Inhibitor of kappa Light Polypeptide Gene Enhancer in B-Cells, Kinase epsilon (IKBKE))

Binding Specificity: pThr501

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This IKKi/IKKe antibody is un-conjugated

Application: Western Blotting (WB), Enzyme Immunoassay (EIA)

Sequence: R-S-R-L-R-pT-L-A-E-V-L

Specificity: This phospho specific polyclonal antibody is specific for phosphorylated pThr501 IKKE. Reactivity with non-phosphorylated IKKE is minimal.

Purification: Immunoaffinity Chromatography against the phosphopeptide and cross adsorption against the non-phosphorylated form of the peptide followed by non-adsorption against a non-specific peptide backbone to further reduce non-specific reactivity.

Immunogen: IKKE phosphopeptide corresponding to a region of the human protein surrounding pThr501 conjugated to KLH. Genename: IKKe

Application Details

Application Notes: This phospho specific polyclonal antibody reacts with phosphorylated human IKKE atpT501 and shows minimal reactivity by Western blot with non-phosphorylated IKKE andminimal reactivity by ELISA against the non-phosphorylated form of the immunizingpeptide. Although not tested, this antibody is likely functional in Immunohistochemistryand Immunoprecipitation.

Restrictions: For Research Use only

Handling

Concentration: 1.15 mg/mL (by UV absorbance at 280 nm)

Buffer: 0.02 M Potassium Phosphate, 0.12 M Sodium Chloride, pH 7.2 with 0.09 % Sodium Azide as preservative.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing. This product is photosensitive and should be protected from light.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for IKKi/IKKe

Target: IKKi/IKKe (IKBKE) (Inhibitor of kappa Light Polypeptide Gene Enhancer in B-Cells, Kinase epsilon (IKBKE))

Alternative Name: IKBKE / IKKE (IKBKE Products)

Synonyms: zgc:100844 antibody, IKBKE antibody, MGC130907 antibody, IKK-E antibody, IKK-i antibody, IKKE antibody, IKKI antibody, AW558201 antibody, IKKepsilon antibody, Ikki antibody, inhibitor of kappa light polypeptide gene enhancer in B-cells, kinase epsilon antibody, inhibitor of nuclear factor kappa B kinase subunit epsilon antibody, inhibitor of nuclear factor kappa B kinase subunit epsilon L homeolog antibody, inhibitor of kappaB kinase epsilon antibody, IKBKE antibody, ikbke antibody, ikbke.L antibody, Ikbke antibody

Background: Nuclear Factor kappa B (NF-κB) is a ubiquitous transcription factor and an essential mediator of gene expression during the activation of immune and inflammatory responses. NF-κB mediates the expression of a great variety of genes in response to extracellular stimuli. NF-κB is associated with IκB proteins in the cytoplasm of the cell, which inhibit NF-κB activity. IκB proteins are phosphorylated by an IκB kinase complex consisting of at least three proteins, IKKa, IKKb, and IKKg. Isolated from a cDNA library of LPS-stimulated mouse macrophage cells, a novel molecule in the IKK complex has been recently identified and designated IKKi and/or IKKe. IKKe is required for the activation of NF-κB by mitogens and T cell receptors but not by TNFa or IL-1. LPS increases the IKKe mRNA level in mouse macrophage cell lines. This protein has significant sequence homology with kinase domains of IKKa and IKKb. Overexpression of wild type IKKe in cells phosphorylates Ser32 and Ser36 of IκBa.Synonyms: I kappa-B kinase epsilon, IKK-E, IKK-I, IKK-epsilon, IKKI, Inducible I kappa-B kinase, Inhibitor of nuclear factor kappa-B kinase subunit epsilon, KIAA0151

Gene ID: 9641

NCBI Accession: NP_001180250

UniProt: Q14164

Pathways: TLR Signaling, Activation of Innate immune Response, Hepatitis C, Toll-Like Receptors Cascades