Recombinant Tubulin-GTP antibody

Catalog No. ABIN1169388

Product Details

Target: Tubulin-GTP

Antibody Type: Recombinant Antibody

Reactivity: Drosophila melanogaster, Human, Mouse, Rat

Host: Human

Clonality: Monoclonal

Conjugate: Un-conjugated

Application: Immunocytochemistry (ICC)

Specificity: Recognizes human, mouse, rat and drosophila tubulin-GTP.

Cross-Reactivity: Fruit Fly (Drosophila melanogaster), Human, Mouse (Murine), Rat (Rattus)

Purification: Purified from HEK 293 cell culture supernatant.

Purity: >95 % (SDS-PAGE)

Immunogen: Full length GTP-lambda-S loaded tubulin from pig brain.

Clone: MB11

Isotype: IgG2

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

anti-Tubulin-GTP, monoclonal antibody (recombinant) (MB11) is composed of human variable regions (VH and VL) (lambda-chain) of immunoglobulin fused to the human lgG2 Fc domain.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: In PBS containing 10 % glycerol and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Short Term Storage: +4°C
Long Term Storage: -20°C
Stable for at least 1 month after receipt when stored at +4°C. Stable for at least 1 year after receipt when stored at -20°C.

Expiry Date: 12 months

References

Van Steenbergen, Boesmans, Li, de Coene, Vints, Baatsen, Dewachter, Ameloot, Clays, Vanden Berghe: "Molecular understanding of label-free second harmonic imaging of microtubules." in: Nature communications, Vol. 10, Issue 1, pp. 3530, (2019) (PubMed).

Nakata, Niwa, Okada, Perez, Hirokawa: "Preferential binding of a kinesin-1 motor to GTP-tubulin-rich microtubules underlies polarized vesicle transport." in: The Journal of cell biology, Vol. 194, Issue 2, pp. 245-55, (2011) (PubMed).

Dimitrov, Quesnoit, Moutel, Cantaloube, Poüs, Perez: "Detection of GTP-tubulin conformation in vivo reveals a role for GTP remnants in microtubule rescues." in: Science (New York, N.Y.), Vol. 322, Issue 5906, pp. 1353-6, (2008) (PubMed).

Target Details

Target: Tubulin-GTP

Background: Anti-Tubulin-GTP, monoclonal antibody (recombinant) (MB11) is an antibody developed by antibody phage display technology using a human naive antibody gene library. These libraries consist of scFv (single chain fragment variable) composed of VH (variable domain of the human immunoglobulin heavy chain) and VL (variable domain of the human immunoglobulin light chain) connected by a polypeptide linker. The antibody fragments are displayed on the surface of filamentous bacteriophage (M13). This scFv was selected by affinity selection on antigen in a process termed panning. Multiple rounds of panning are performed to enrich for antigen-specific scFv-phage. Monoclonal antibodies are subsequently identified by screening after each round of selection. The selected monoclonal scFv is cloned into an appropriate vector containing a Fc portion of interest and then produced in mammalian cells to generate an IgG like scFv-Fc fusion protein.