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Details for Product No. ABIN116945

Prostaglandin-Endoperoxide Synthase 2 (Prostaglandin G/H Synthase and Cyclooxygenase) (PTGS2) (C-Term) antibody

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Antigen
Synonyms COX2, COX-2, PHS-2, PGG/HS, PGHS-2, hCox-2, GRIPGHS, Cox-2, Pghs2, TIS10, Cox2, CEF147, PGHS2, PHSII, cox-2, cox2, gripghs, pgg/hs, pghs-2, phs-2, PTGS2, hcox-2
Epitope
»Alternatives C-Term
Reactivity
»Alternatives Human, Mouse (Murine), Rat (Rattus)
Host
»Alternatives Rabbit
Clonality Polyclonal
Conjugate
»Alternatives Un-conjugated
Application
»Alternatives ELISA, Western Blotting (WB)
Pubmed 5 references available
Catalog no. ABIN116945
Quantity 0.1 mL
Price
379.50 $   Plus shipping costs $45.00
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Immunogen GST fusion protein corresponding to the carboxy-terminus of Human Cox-2.
Specificity This antiserum is directed against Human Cox-2 and is useful in determining its presence in Western blotting and ELISA experiments. The antibody does not cross react with Cox-1. Cox-2 is involved in the response of cells to growth factors, tumor promoters, and cytokines that induce its expression. Given its role in synthesizing prostaglandins, Cox-2 is therefore of interest in studying immune response regulation. This antibody can detect Cox-2 in Human, Mouse and Rat.
Purification Serum
Alternative Name Cyclooxygenase 2
Background Cox-2 (Cyclooxygenase-2) is an inducible enzyme that is normally absent from cells, however, in response to growth factors, tumor promoters and some cytokines, it undergoes a rapid and transient expression. COX-2, also known as prostaglandin H synthase, was identified less than a decade ago. Its discovery was followed by a period of discovery and drug development to create a "super aspirin" and led to insights into arthritis, Alzheimer's disease, colorectal cancer and regulation of brain and kidney function in search of better treatments for degenerative and inflammatory diseases. Two different isoforms of the enzyme are known, Cox-1 and Cox-2.
Alternate names: COX2, Cyclooxygenase-2, PGH synthase 2, PGHS-2, PGHS2, PHS II, PTGS2, Prostaglandin G/H synthase 2, Prostaglandin H2 synthase 2, Prostaglandin-endoperoxide synthase 2
Gene ID 5743
NCBI Accession NP_000954.1
UniProt P35354
Application Notes This antibody has been tested for use in Immunoblotting and ELISA. The antibodyrecognizes Cox-2 at 70kDa in Sf9 cell lines transfected with Cox-2 as well as in WISH cellsinduced with IL-1b. Reactivity in other immunoassays is unknown. Recommended Dilutions: This product was assayed by Immunoblot using HRP Goatanti-Rabbit IgG and ECL for detection. A working dilution of 1/5,000-1/10,000 is suggestedfor this assay. For ELISA a working dilution of 1/10,000-1/50,000 is suggested usingProtein A HRP and TMB as a substrate for 30 minutes at RT. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Format Liquid
Concentration 55 mg/mL (by Refractometry)
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
Expiry Date 12 months
Background publications Gierse, McDonald, Hauser et al.: "A single amino acid difference between cyclooxygenase-1 (COX-1) and -2 (COX-2) reverses the selectivity of COX-2 specific inhibitors." in: The Journal of biological chemistry, Vol. 271, Issue 26, pp. 15810-4, 1996 (PubMed).

Guan, Buckman, Miller et al.: "Interleukin-1beta-induced cyclooxygenase-2 expression requires activation of both c-Jun NH2-terminal kinase and p38 MAPK signal pathways in rat renal mesangial cells." in: The Journal of biological chemistry, Vol. 273, Issue 44, pp. 28670-6, 1998 (PubMed).

Hittelman, Burakov, Iñiguez-Lluhí et al.: "Differential regulation of glucocorticoid receptor transcriptional activation via AF-1-associated proteins." in: The EMBO journal, Vol. 18, Issue 19, pp. 5380-8, 1999 (PubMed).

Tong, Tai: "Induction of NAD(+)-linked 15-hydroxyprostaglandin dehydrogenase expression by androgens in human prostate cancer cells." in: Biochemical and biophysical research communications, Vol. 276, Issue 1, pp. 77-81, 2000 (PubMed).

Kelloff, Sullivan, Baker et al.: "Workshop on imaging science development for cancer prevention and preemption." in: Cancer biomarkers : section A of Disease markers, Vol. 3, Issue 1, pp. 1-33, 2007 (PubMed).

Alternatives for antigen "Prostaglandin-Endoperoxide Synthase 2 (Prostaglandin G/H Synthase and Cyclooxygenase) (PTGS2)", type "Antibodies"
Hosts (112), (45), (15)
Reactivities (137), (59), (45), (19), (14), (14), (6), (2), (1)
Applications (116), (72), (60), (24), (17), (16), (15), (10), (10), (4), (3), (3), (2), (2), (1), (1)
Conjugates (8), (3), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Epitopes (43), (10), (3), (3), (3), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
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