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CREB1 antibody (CAMP Responsive Element Binding Protein 1) (N-Term)

Details for Product anti-CREB1 Antibody No. ABIN116948, Supplier: Log in to see
Antigen
  • 2310001E10Rik
  • 3526402H21Rik
  • AV083133
  • Creb
  • CREB
  • creb
  • Creb-1
  • creb1
  • Creb1
  • CREB1
  • zgc:55598
Alternatives
anti-Human CREB1 antibody for Western Blotting
Epitope
N-Term
112
36
34
33
28
21
17
16
16
13
10
7
7
5
4
4
3
3
3
3
3
3
3
3
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine), Rat (Rattus)
492
251
233
27
25
19
18
15
14
9
9
9
2
2
1
1
1
Host
Rabbit
424
74
10
1
Clonality
Polyclonal
Conjugate
This CREB1 antibody is un-conjugated
17
15
14
7
6
6
6
5
3
3
3
3
3
3
3
1
1
1
1
1
1
Application
Electrophoretic Mobility-Shift Assay (EMSA), Enzyme Immunoassay (EIA), Immunoprecipitation (IP), Western Blotting (WB)
393
224
187
73
65
46
36
34
26
10
9
4
3
2
2
1
1
Supplier
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Immunogen CREB-1 (p43) peptide corresponding to a region near the N-terminus of the Human protein conjugated to Keyhole Limpet Hemocyanin (KLH).
Specificity This product was prepared from monospecific antiserum by delipidation and Immunoadsorption against an E.coli lysate immobilized on agarose beads. This antibody may react non-specifically with other proteins. A partial cross-reactivity is observed against CREM-1 protein.
Alternative Name CREB1 (CREB1 Antibody Abstract)
Background Cyclic AMP Response Element Binding protein (CREB) is a basic / leucine zipper transcription factor that binds the cyclic AMP response element (CRE) and activates transcription in response to a variety of extracellular signals including neurotransmitters, hormones, membrane depolarization, and growth and neurotrophic factors. Activation of CREB is dependent upon the phosphorylation of serine 133. Phosphorylation occurs via p44 / 42 MAP kinase and p90RSK and also via p38 MAP kinase and MSK 1. Although CREB will bind DNA independent of its phosphorylation state, only the phosphorylated form is competent as a transcription factor. CREB binding protein (CBP), a transcriptional coactivator that directly interacts with CREB, binds to CREB in the region of serine 133. CREB appears to play an important role in learning and memory. CREB knock out mice show diminished learning ability.Synonyms: CREB-1, Cyclic AMP-responsive element-binding protein 1, cAMP-responsive element-binding protein 1
Gene ID 1385
NCBI Accession NP_004370
UniProt P16220
Pathways TCR Signaling, TLR Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway
Application Notes Western Blot: This antibody was assayed against a lysate of 1 x 10^6 Raji B cells byImmunoblot and found to be reactive against CREB-1 (p43), showing a 46 kDa band at adilution of 1/500-1/1,000 followed by reaction with Peroxidase conjugated anti-Rabbit IgG(H&L) [Goat] (Cat#R1364HRP)ELISA: 1/5,000-1/25,000. Immunoprecipitation. Gelshift: This antibody was tested in Gel Supershift Assay and found to be reactive againstCREB-1 (p43) using 0.5-1.0 μL per assay.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Format Liquid
Concentration 2.56 mg/mL (by UV absorbance at 280 nm)
Buffer 0.02 M Potassium Phosphate, 0.12 M Sodium Chloride, pH 7.2, 0.01 % (w/v) Sodium Azide, None
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store lyophilized (preferably in a desiccator) at 2-8 °C and reconstituted (in aliquots) at -20 °C.
Supplier Images
Western Blotting (WB) image for anti-CREB1 antibody (CAMP Responsive Element Binding Protein 1) (N-Term) (ABIN116948) Anti-CREB is shown to detect CREB-1 present in Raji B cell nuclear extract lysates. D...
Background publications Moreno, Beresford, Louis-Plence, Morris, Boss: "CREB regulates MHC class II expression in a CIITA-dependent manner." in: Immunity, Vol. 10, Issue 2, pp. 143-51, 1999 (PubMed).