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CREB1 antibody (CAMP Responsive Element Binding Protein 1) (pSer133)

Details for Product anti-CREB1 Antibody No. ABIN116949, Supplier: Log in to see
Antigen
  • creb
  • CREB1
  • Creb1
  • CREB
  • 2310001E10Rik
  • 3526402H21Rik
  • AV083133
  • Creb
  • Creb-1
  • creb1
  • zgc:55598
  • cAMP responsive element binding protein 1
  • cAMP responsive element binding protein 1a
  • cyclic AMP response element binding protein
  • CREB1
  • creb1
  • Creb1
  • LOC100351999
  • creb1a
  • CREB
Alternatives
anti-Human CREB1 antibody for Western Blotting
Epitope
pSer133
112
38
36
35
20
18
17
16
15
15
8
7
7
6
5
5
5
3
3
3
3
3
3
3
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine), Rat (Rattus)
508
326
293
41
41
36
29
27
25
24
21
14
13
10
7
7
3
1
1
1
1
1
1
Host
Rabbit
424
84
11
1
Clonality
Polyclonal
Conjugate
This CREB1 antibody is un-conjugated
17
14
14
7
6
6
6
5
3
3
3
3
3
3
3
3
3
1
1
1
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Application
Enzyme Immunoassay (EIA), Immunoprecipitation (IP), Western Blotting (WB)
405
212
181
83
83
43
42
30
29
11
10
10
4
3
3
2
1
1
1
1
Options
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Immunogen CREB phospho peptide corresponding to a region of the human protein conjugated to Keyhole Limpet Hemocyanin (KLH).
Sequence Q-K-R-R-E-I-L-S-R-R-P-pS-Y-R-K-I-L-N-D-L-S-S-D-A-P correspond ing to aa 122-147
Specificity This phospho specific polyclonal antibody reacts with phosphorylated human CREB at pS133 and shows minimal reactivity by western blot with non-phosphorylated CREB and minimal reactivity by ELISA against the non-phosphorylated form of the immunizing peptide. This product was affinity purified from monospecific antiserum by immunoaffinity purification. Antiserum was first sequentially pre-adsorbed against a control E.coli lysate, nickel-purified recombinant CREB and the non-phosphorylated form of the immunizing peptide. The resultant depleted antiserum was then purified against the phosphorylated form of the immunizing peptide. This phospho specific polyclonal antibody is specific for phosphorylated pS133 of human CREB. Reactivity with non-phosphorylated CREB is minimal. This antibody does show cross reactivity with pS133 phosphorylated CREB from mouse and rat.
Purification Affinity Purified
Alternative Name CREB1 (CREB1 Antibody Abstract)
Background The CREB (Cyclic AMP-response-element-binding-protein 1) gene encodes a transcription factor that is a member of the leucine zipper family of DNA binding proteins. This protein binds as a homodimer to the cAMP-responsive element (CRE element TGANNTCA), an octameric palindrome. Phosphorylation by cAMPdependent protein kinase (PKA) at serine-119 is required for interaction with DNA and phosphorylation at serine-133 allows CREB to interact with CBP (CREB binding protein) leading to interaction with RNA polymerase II. Alternate splicing of this gene results in two transcript variants encoding different isoforms.Synonyms: CREB-1, Cyclic AMP-responsive element-binding protein 1, cAMP-responsive element-binding protein 1
Gene ID 1385
NCBI Accession NP_004370
UniProt P16220
Pathways TCR Signaling, TLR Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Thyroid Hormone Synthesis, Activation of Innate immune Response, Myometrial Relaxation and Contraction, Regulation of Cell Size, Toll-Like Receptors Cascades
Application Notes Western Blot: 1: 1,000. ELISA. Immunoprecipitation.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Format Liquid
Concentration 0.064 mg/mL (by UV absorbance at 280 nm)
Buffer 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 containing 0.1 % (w/v) Sodium Azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
Supplier Images
 image for anti-CREB1 antibody (CAMP Responsive Element Binding Protein 1) (pSer133) (ABIN116949) Anti-CREB pS133 was used to detect phosphorylated CREB by immunoblot. Recombinant His...
 image for anti-CREB1 antibody (CAMP Responsive Element Binding Protein 1) (pSer133) (ABIN116949) Anti-CREB pS133 was used to detect phosphorylated CREB by immunoblot. Recombinant His...
Background publications Arthur, Cohen: "MSK1 is required for CREB phosphorylation in response to mitogens in mouse embryonic stem cells." in: FEBS letters, Vol. 482, Issue 1-2, pp. 44-8, 2000 (PubMed).

Kim, Lu, Quinn: "Distinct cAMP response element-binding protein (CREB) domains stimulate different steps in a concerted mechanism of transcription activation." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 97, Issue 21, pp. 11292-6, 2000 (PubMed).

Moreno, Beresford, Louis-Plence, Morris, Boss: "CREB regulates MHC class II expression in a CIITA-dependent manner." in: Immunity, Vol. 10, Issue 2, pp. 143-51, 1999 (PubMed).

Kwok, Laurance, Lundblad, Goldman, Shih, Connor, Marriott, Goodman: "Control of cAMP-regulated enhancers by the viral transactivator Tax through CREB and the co-activator CBP." in: Nature, Vol. 380, Issue 6575, pp. 642-6, 1996 (PubMed).

Barton, Muthusamy, Chanyangam, Fischer, Clendenin, Leiden: "Defective thymocyte proliferation and IL-2 production in transgenic mice expressing a dominant-negative form of CREB." in: Nature, Vol. 379, Issue 6560, pp. 81-5, 1996 (PubMed).

Lee, Yun, Hoeffler, Habener: "Cyclic-AMP-responsive transcriptional activation of CREB-327 involves interdependent phosphorylated subdomains." in: The EMBO journal, Vol. 9, Issue 13, pp. 4455-65, 1991 (PubMed).

Dwarki, Montminy, Verma: "Both the basic region and the 'leucine zipper' domain of the cyclic AMP response element binding (CREB) protein are essential for transcriptional activation." in: The EMBO journal, Vol. 9, Issue 1, pp. 225-32, 1990 (PubMed).