ATG12 antibody

Catalog No. ABIN116953

Product Details anti-ATG12 Antibody

Target: ATG12 (Autophagy Related 12 (ATG12))

Reactivity: Saccharomyces cerevisiae

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ATG12 antibody is un-conjugated

Application: Western Blotting (WB), Enzyme Immunoassay (EIA)

Specificity: Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum.

Cross-Reactivity (Details): Species reactivity (tested):S. cerevisiae, Yeast

Purification: A multi-step process which includes Delipidation, Salt Fractionation and Ion exchange chromatography followed by extensive dialysis against the buffer stated below.

Immunogen: This purified antibody was prepared from rabbit serum after repeated immunizations with recombinant yeast APG12 protein.

Application Details

Application Notes: This purified polyclonal antibody reacts with yeast APG12 by Western blot and ELISA. This antibody using the specified conditions may recognize other prominent intrinsicbands (UBLs or conjugates).
Other intrinsic bands are readily detectable at lower dilutions. A 22.1 kDa band corresponding to yeast APG12 is detected. Most yeast cell lysates can be used as a positive control without induction or stimulation. Recommended Dilutions: ELISA: 1/1,000-1/5,000. Western Blot: 1/200. Although not tested, this antibody is likely functional in Immunohistochemistry andImmunoprecipitation.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Reconstitution: Restore with 0.1 mL of deionized water or equivalent.

Concentration: 5.0 mg/mL (by UV absorbance at 280 nm)

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 with 0.01 % (w/v) Sodium Azide as preservative

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing. Dilute only prior to immediate use

Storage: 4 °C/-20 °C

Storage Comment: Store vial at 2-8 °C prior to restoration. For extended storage add glycerol to 50% and then aliquot contents and freeze at -20 °C or below. Centrifuge product if not completely clear after standing at room temperature. This antibody is stable for one month at 2-8 °C as an undiluted liquid.

Target Details for ATG12

Target: ATG12 (Autophagy Related 12 (ATG12))

Alternative Name: ATG12 / APG12 (ATG12 Products)

Synonyms: APG12 antibody, APG12L antibody, FBR93 antibody, HAPG12 antibody, Apg12l antibody, AUTOPHAGY 12 antibody, AUTOPHAGY 12 B antibody, CG10861 antibody, Dmel\\CG10861 antibody, atg12 antibody, ATG12 antibody, apg12 antibody, DDBDRAFT_0185323 antibody, DDBDRAFT_0191412 antibody, DDB_0185323 antibody, DDB_0191412 antibody, 4931423H11Rik antibody, A330058M13Rik antibody, Atg12l antibody, AO090001000593 antibody, autophagy related 12 antibody, Ubiquitin-like superfamily protein antibody, Atg12p antibody, autophagy protein antibody, ubiquitin-like protein ATG12B antibody, autophagy associated protein Atg12 antibody, Autophagy-related 12 antibody, ATG12 autophagy related 12 homolog (S. cerevisiae) antibody, ATG12 autophagy related 12 homolog (S. cerevisiae) pseudogene antibody, autophagy associated ubiquitin-like modifier Atg12 antibody, hypothetical protein antibody, Autophagy-related protein 12 (Autophagy-related ubiquitin-like modifier ATG12) antibody, autophagy protein 12 antibody, autophagy related 12 S homeolog antibody, autophagy-related protein 12 antibody, ATG12 antibody, Atg12 antibody, APG12B antibody, APG12 antibody, LOC9321066 antibody, apg12 antibody, atg12 antibody, LOC743425 antibody, CAALFM_C113950CA antibody, atg12.S antibody, ANI_1_922094 antibody, AOR_1_1064164 antibody

Background: Ubiquitin-like proteins fall into two classes: the first class, ubiquitin-like modifiers (UBLs) function as modifiers in a manner analogous to that of ubiquitin. Examples of UBLs are SUMO, Rub1 (also called Nedd8), Apg8 and Apg12. Proteins of the second class include parkin, RAD23 and DSK2, are designated ubiquitin-domain proteins (UDPs). These proteins contain domains that are related to ubiquitin but are otherwise unrelated to each other. In contrast to UBLs, UDPs are not conjugated to other proteins. In yeast, autophagy, the delivery of cytoplasmic components to the lysosome/vacuole for degradation, requires a ubiquitin-like protein conjugation system, in which Apg12 is covalently bound to Apg12-Apg5 and Apg16.Synonyms: APG12-like, APG12L, Autophagy-related protein 12, Ubiquitin-like protein ATG12

Gene ID: 852518

NCBI Accession: NP_009776

UniProt: P38316

Pathways: Autophagy